FastQCFastQC Report
Mon 6 Feb 2017
SRR2077353_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077353_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555186
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77370.30279596084198956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35650.13952017583064405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35260.1379938681567604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17950.026.1357481
GTATCAA44350.023.6876071
TATCAAC56900.018.1910252
TCAACGC58350.017.6239574
ATCAACG58950.017.44563
CAACGCA59750.017.1708015
AACGCAG61050.016.7999046
TATACCG1351.6419472E-716.2955885
ATAGGAC3700.014.6005333
ACGCAGA70050.014.4416717
CGCAGAG71350.013.9543768
TACACCG2159.058567E-1013.9528815
GTATAAC3100.013.5812931
AGAGTAC69000.013.44474311
GTATTAT6100.013.1466071
GTATAGG4450.013.0653641
GAGTACT36500.013.036712
CAGAGTA72350.012.96038410
GCAGAGT75550.012.596689
AGTACTT38950.012.52466113
Produced by FastQC (version 0.11.5)