FastQCFastQC Report
Mon 6 Feb 2017
SRR2077353_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077353_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555186
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26800.042.4616361
GTATCAA61450.039.1827771
TATCAAC85200.028.0841922
ATCAACG85300.027.8452593
TCAACGC85700.027.6748314
CAACGCA87500.027.0257325
AACGCAG90650.026.1610556
ACGCAGA104200.022.3483877
CGCAGAG107100.021.7016858
AGAGTAC106450.021.12192311
GAGTACT75350.020.73225212
CAGAGTA112550.020.160610
GCAGAGT117300.019.6087939
GTACATG68700.019.3924261
AGTACTT81450.019.221913
TACATGG70150.018.7848782
GTACTTT82450.018.69415914
ACATGGG73600.017.148783
GTGGTAT10600.017.0198251
TACTTTT92800.016.94427115
Produced by FastQC (version 0.11.5)