FastQCFastQC Report
Mon 6 Feb 2017
SRR2077352_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077352_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811821
Sequences flagged as poor quality0
Sequence length46
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62040.34241793201425524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28570.15768665889180003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27910.15404391493420155No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21810.12037612987154912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.027.6565231
GTATCAA48550.023.4016671
TAGGGCG1401.8189894E-1221.42950231
AGTATCG1101.573244E-820.0030785
TGGTCGG2750.018.90208610
GGTCGGT2800.018.56454811
TAGTCGG2700.018.52597240
AACGCGG659.34266E-418.4694835
CGCTTAC659.3913014E-418.45520818
TTAGTCG2800.017.86531839
TATCAAC64450.017.4240322
ATCAACG65050.017.0413193
TCAACGC65500.016.9515134
GTCGGTT3100.016.76797912
CCCTATA3500.016.5858862
CAACGCA66900.016.5645835
GCGTTTA1951.0913936E-1116.41731635
CTGGTCG3200.016.243989
CCTATAC3850.016.1117133
AACGCAG68800.016.0405656
Produced by FastQC (version 0.11.5)