##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077352_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1811821
Sequences flagged as poor quality	0
Sequence length	46
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.810983535349244	31.0	30.0	34.0	25.0	34.0
2	29.908901044860393	31.0	30.0	34.0	25.0	34.0
3	29.985578597444228	31.0	31.0	34.0	25.0	34.0
4	33.341261084842266	37.0	35.0	37.0	27.0	37.0
5	33.42421685144394	37.0	35.0	37.0	27.0	37.0
6	33.4845682879269	37.0	35.0	37.0	27.0	37.0
7	33.376836343104536	37.0	35.0	37.0	27.0	37.0
8	33.28378741608581	37.0	35.0	37.0	26.0	37.0
9	34.672841301651765	39.0	35.0	39.0	26.0	39.0
10	34.62640956253405	39.0	35.0	39.0	26.0	39.0
11	34.6297321865681	39.0	35.0	39.0	26.0	39.0
12	34.56157203167421	39.0	35.0	39.0	25.0	39.0
13	34.507482251282	39.0	35.0	39.0	25.0	39.0
14	35.49787644585199	40.0	36.0	41.0	23.0	41.0
15	35.63153755255072	40.0	36.0	41.0	25.0	41.0
16	35.608231166323826	40.0	36.0	41.0	25.0	41.0
17	35.64289353087308	40.0	36.0	41.0	25.0	41.0
18	35.62675672707182	40.0	36.0	41.0	25.0	41.0
19	35.74745132107421	40.0	36.0	41.0	25.0	41.0
20	35.740648220767945	40.0	36.0	41.0	25.0	41.0
21	35.76037423122924	40.0	36.0	41.0	25.0	41.0
22	35.709126894985765	40.0	36.0	41.0	25.0	41.0
23	35.67357868133772	40.0	36.0	41.0	25.0	41.0
24	35.70993105831095	40.0	36.0	41.0	25.0	41.0
25	35.65792702479991	40.0	36.0	41.0	25.0	41.0
26	35.69009576553092	40.0	36.0	41.0	26.0	41.0
27	35.666621040378715	40.0	36.0	41.0	25.0	41.0
28	35.5994091027756	40.0	36.0	41.0	25.0	41.0
29	35.57693558028083	40.0	36.0	41.0	25.0	41.0
30	35.48309132083136	40.0	36.0	41.0	25.0	41.0
31	35.44183779744246	40.0	36.0	41.0	25.0	41.0
32	35.403716481926196	40.0	35.0	41.0	25.0	41.0
33	35.35097396486739	40.0	35.0	41.0	25.0	41.0
34	35.30898637337794	40.0	35.0	41.0	25.0	41.0
35	35.287690119498556	40.0	35.0	41.0	25.0	41.0
36	35.22320361669282	40.0	35.0	41.0	24.0	41.0
37	35.148592493408565	40.0	35.0	41.0	24.0	41.0
38	35.09676783744089	40.0	35.0	41.0	24.0	41.0
39	35.0256471251851	40.0	35.0	41.0	23.0	41.0
40	35.006189905073406	40.0	35.0	41.0	23.0	41.0
41	34.8801095693228	40.0	35.0	41.0	22.0	41.0
42	34.82141778906415	40.0	35.0	41.0	22.0	41.0
43	34.725924360077514	40.0	35.0	41.0	20.0	41.0
44	34.64319709286955	40.0	35.0	41.0	18.0	41.0
45	34.53698185416771	40.0	35.0	41.0	16.0	41.0
46	34.42941383282344	40.0	35.0	41.0	15.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5108878597405315
1101	2	0.6431163910841455
1101	3	0.7638768945614913
1101	4	0.7314944522155074
1101	5	0.599597920428657
1101	6	0.6512770103610066
1101	7	0.7321968130768326
1101	8	0.8005778876406282
1101	9	0.8663554971713836
1101	10	0.7165783832735997
1101	11	0.7451190020248362
1101	12	0.8201370444593863
1101	13	0.8173436246342902
1101	14	1.0722943249839219
1101	15	0.8833590593148841
1101	16	0.7780604431187257
1101	17	0.8789649968579525
1101	18	0.8432260825020492
1101	19	0.7727010233063041
1101	20	0.7471645250850898
1101	21	0.818238629436415
1101	22	0.9248626089624921
1101	23	0.9014435980934863
1101	24	0.7939669478532565
1101	25	0.9087683776440727
1101	26	0.867083571522933
1101	27	0.8840063087436363
1101	28	0.7942603848069751
1101	29	0.8115255573767755
1101	30	1.033546744962237
1101	31	0.9774655428434329
1101	32	1.0504306165445385
1101	33	1.1602355994279492
1101	34	0.9613766790197431
1101	35	0.9924666481165119
1101	36	0.8362208216937503
1101	37	0.8104429685404781
1101	38	0.8929663428314498
1101	39	0.9484143942222758
1101	40	1.0222154088208484
1101	41	0.9435850986928358
1101	42	0.8532183825307484
1101	43	0.82813894772098
1101	44	0.9356287690515543
1101	45	0.8199085722447563
1101	46	0.7300599136466559
1102	1	0.4137514966718321
1102	2	0.4923851833112387
1102	3	0.4488768321184686
1102	4	0.486355641380392
1102	5	0.4257128405640316
1102	6	0.35911236060390905
1102	7	0.43893316615464784
1102	8	0.4093099196614034
1102	9	0.5287697941254095
1102	10	0.4847961345755394
1102	11	0.4022443751218816
1102	12	0.5391934055299075
1102	13	0.6176383556922005
1102	14	0.921875332314734
1102	15	0.7695753869159461
1102	16	0.705507897316771
1102	17	0.6695498381902354
1102	18	0.5278201404241898
1102	19	0.6593731849587954
1102	20	0.6099772085114594
1102	21	0.4078256312972215
1102	22	0.5685127781830772
1102	23	0.6403943055729115
1102	24	0.6236536087751645
1102	25	0.6116332634357846
1102	26	0.6566943017316191
1102	27	0.5938273470461795
1102	28	0.6616189201434821
1102	29	0.6873139005136579
1102	30	0.7098608583337835
1102	31	0.6327925569430661
1102	32	0.6065606478909373
1102	33	0.7000492694540483
1102	34	0.7363570094682075
1102	35	0.7291194524325775
1102	36	0.7405331367062971
1102	37	0.690770919285427
1102	38	0.6578371482215317
1102	39	0.7465048866479336
1102	40	0.7146460658463099
1102	41	0.794596051198674
1102	42	0.7312484301123305
1102	43	0.7169653935891276
1102	44	0.6828032223629066
1102	45	0.761518103547445
1102	46	0.6391678526523137
1103	1	0.4979551673457472
1103	2	0.48408678380492987
1103	3	0.546917061506619
1103	4	0.4665113260640936
1103	5	0.5564864298480003
1103	6	0.5356215568985903
1103	7	0.4491632881905474
1103	8	0.44207789398077324
1103	9	0.6127571617920964
1103	10	0.4711841982149707
1103	11	0.4922476701773135
1103	12	0.49548890752669905
1103	13	0.5325535770952641
1103	14	0.7500167992353752
1103	15	0.6773915317367525
1103	16	0.7699384127983322
1103	17	0.7577921058776838
1103	18	0.7015138321906278
1103	19	0.6773539666742963
1103	20	0.7593155679185202
1103	21	0.7675189623605618
1103	22	0.741880529841076
1103	23	0.7604314811666839
1103	24	0.750307487417885
1103	25	0.6644073346476063
1103	26	0.6993386773037571
1103	27	0.7422970775498712
1103	28	0.698906082292595
1103	29	0.7419611617105346
1103	30	0.8146413924893068
1103	31	0.7838543580993971
1103	32	0.7674435860701649
1103	33	0.9016735327090686
1103	34	0.8166782194339532
1103	35	0.7917436298200542
1103	36	0.75336023186685
1103	37	0.7975437883516889
1103	38	0.7630627674111707
1103	39	0.7917490579801196
1103	40	0.7110769176671639
1103	41	0.8191098968195334
1103	42	0.7410373296678046
1103	43	0.9425997363265921
1103	44	0.9332880471322156
1103	45	0.8580134600151013
1103	46	0.8892558743087591
1104	1	0.36002883890953896
1104	2	0.3971682601598623
1104	3	0.43363595426326285
1104	4	0.3427544509042022
1104	5	0.2745834609666673
1104	6	0.4091934194502116
1104	7	0.3926933287562733
1104	8	0.35059972318443755
1104	9	0.3187941117793045
1104	10	0.43053510089377056
1104	11	0.4046156488346213
1104	12	0.38360881396970825
1104	13	0.4561679240236032
1104	14	0.5957616805532098
1104	15	0.5367973671714594
1104	16	0.5429516400577725
1104	17	0.44718848511242015
1104	18	0.3969131282290732
1104	19	0.35792198964327326
1104	20	0.5908981337921446
1104	21	0.4401251618818094
1104	22	0.4774092890231856
1104	23	0.42746360483733525
1104	24	0.5504344837267681
1104	25	0.3359243009890207
1104	26	0.37786699336925267
1104	27	0.43818831297726746
1104	28	0.3803023792995006
1104	29	0.5567032975326285
1104	30	0.5303366858599148
1104	31	0.5239196378567357
1104	32	0.5457816360829568
1104	33	0.615914421409137
1104	34	0.5709243729989879
1104	35	0.47552624685766176
1104	36	0.5098602272986383
1104	37	0.4968860228471783
1104	38	0.5740652502346961
1104	39	0.42820071030259754
1104	40	0.42856580549013756
1104	41	0.520199758128598
1104	42	0.5465068505536763
1104	43	0.5825891837556014
1104	44	0.5516270222080522
1104	45	0.49091161400041017
1104	46	0.5244484130472031
1105	1	0.32747341404498087
1105	2	0.24304124633037816
1105	3	0.47782213547227315
1105	4	0.470904401004006
1105	5	0.6198817037667794
1105	6	0.6261568140895051
1105	7	0.49138879496670995
1105	8	0.6483591151148644
1105	9	0.8192463323381816
1105	10	0.6732550182020134
1105	11	0.7889897577596301
1105	12	0.901768922324429
1105	13	0.9479388464644316
1105	14	1.1321910539492777
1105	15	0.9220491849644503
1105	16	0.973352000052067
1105	17	0.7772916715965934
1105	18	0.836259881151797
1105	19	0.763323240712559
1105	20	0.8187549202192912
1105	21	0.8285024252122923
1105	22	0.8178110747374703
1105	23	0.7932142464113241
1105	24	0.8285211675020747
1105	25	0.6996165593294776
1105	26	0.8549284953652574
1105	27	0.8212124432880756
1105	28	0.9982851637306354
1105	29	0.8166187345158065
1105	30	0.9818956215599428
1105	31	0.9598780781190683
1105	32	0.8996520203144982
1105	33	0.9024952775539887
1105	34	0.7672281939285597
1105	35	0.9562522947618532
1105	36	1.016975461116715
1105	37	0.988890852473638
1105	38	0.9400943527439622
1105	39	0.9845233329145202
1105	40	0.7943810500011139
1105	41	0.8490999933723984
1105	42	0.8505594596212518
1105	43	0.881054752617878
1105	44	0.8325517798356543
1105	45	0.874860234616321
1105	46	0.9083346584136152
1106	1	-0.1567497232905346
1106	2	-0.09518650635914838
1106	3	-0.1076240817278773
1106	4	-0.05221947859007514
1106	5	-0.15741116343348693
1106	6	-0.14353014090416139
1106	7	-0.020628007530177683
1106	8	-0.07462349110127064
1106	9	-0.08477674001102287
1106	10	-0.005430661380131596
1106	11	-0.14997091447287403
1106	12	-0.1144993440221711
1106	13	-0.1481198666740724
1106	14	0.22364270214789883
1106	15	0.1726581552851556
1106	16	0.09408616848154594
1106	17	0.14756800707623796
1106	18	0.03433410921836355
1106	19	-0.04552197030504601
1106	20	-0.057553785735358076
1106	21	-0.12684200773797727
1106	22	-0.19319390865123154
1106	23	-0.1498712050556179
1106	24	-0.06995126747293767
1106	25	-0.05020757424169631
1106	26	-0.1069031016842743
1106	27	0.007327063438694381
1106	28	0.005135112780486395
1106	29	-0.03549804521377098
1106	30	0.04538202085438314
1106	31	0.008288870045731755
1106	32	0.04692654656653161
1106	33	0.03648878002855582
1106	34	0.04266209909717844
1106	35	-0.17172596798176443
1106	36	-0.1279270096891807
1106	37	-0.19006891901329226
1106	38	-0.20315016597054836
1106	39	-0.12212632458112438
1106	40	-0.07449401824555935
1106	41	0.042180885851003325
1106	42	0.030287766020634876
1106	43	0.0033279249885254103
1106	44	0.12351125070565416
1106	45	0.04639507191846093
1106	46	0.022173011607556248
1107	1	0.2516546864838425
1107	2	0.16025470520951401
1107	3	0.27285323337765277
1107	4	0.29383150487360865
1107	5	0.34886708320667026
1107	6	0.18414442578335866
1107	7	0.25471834295422013
1107	8	0.24022669906514693
1107	9	0.419253582023849
1107	10	0.3197142230305161
1107	11	0.257101149720917
1107	12	0.2930237725805469
1107	13	0.2888174434446711
1107	14	0.4408826696337016
1107	15	0.41509435591636645
1107	16	0.5191137472479568
1107	17	0.3354966386051146
1107	18	0.49571536483567513
1107	19	0.4825980990190786
1107	20	0.45182979865180073
1107	21	0.28888329881023367
1107	22	0.3160266511740417
1107	23	0.30556779194043315
1107	24	0.3625332842155231
1107	25	0.2835645379656313
1107	26	0.14966500716276698
1107	27	0.3713108821169584
1107	28	0.2999863782152943
1107	29	0.2504819892134975
1107	30	0.3139319013675177
1107	31	0.44426217403998436
1107	32	0.43456480226775795
1107	33	0.47036464362849273
1107	34	0.33900846785846994
1107	35	0.2493900885634801
1107	36	0.3160370325888735
1107	37	0.36370217128884264
1107	38	0.2428547517147237
1107	39	0.3411020980441819
1107	40	0.4289902648926187
1107	41	0.487788914770654
1107	42	0.42047104591740236
1107	43	0.3609931411101357
1107	44	0.3087907998079942
1107	45	0.3237642877316276
1107	46	0.37456180837823183
1108	1	0.3204478928947694
1108	2	0.3647886605927617
1108	3	0.22412925385582483
1108	4	0.2595158145405705
1108	5	0.19005766201071594
1108	6	0.07846783376138688
1108	7	0.06008255717491551
1108	8	0.0883507895200637
1108	9	0.131441727643832
1108	10	0.23077860738727907
1108	11	0.24858687368819687
1108	12	0.04046519288490913
1108	13	0.029042414728969845
1108	14	0.24135667991659204
1108	15	0.23882579217782762
1108	16	0.18068599516345074
1108	17	0.20230928343174526
1108	18	0.2868119854992486
1108	19	0.2586982660629289
1108	20	0.34538788423777333
1108	21	0.23642521599436606
1108	22	0.17474066475529781
1108	23	0.3758720591281417
1108	24	0.3725697014502245
1108	25	0.260792202695157
1108	26	0.23544553614999586
1108	27	0.1733590858318692
1108	28	0.33116579284673975
1108	29	0.44253655128867564
1108	30	0.11139371094267858
1108	31	0.26326742242510903
1108	32	0.40294796852502657
1108	33	0.20267793363220932
1108	34	0.4425089159070623
1108	35	0.2810855160198713
1108	36	0.3017186752232561
1108	37	0.3261971222869491
1108	38	0.21482990837067462
1108	39	0.29636462359518845
1108	40	0.40056613971473354
1108	41	0.2828087790096774
1108	42	0.3297478742358777
1108	43	0.4192313406183459
1108	44	0.31328429875503616
1108	45	0.29958911909335484
1108	46	0.2894650446998668
1109	1	0.33560852974109423
1109	2	0.25831453770073054
1109	3	0.40853267601166365
1109	4	0.4366043975993037
1109	5	0.29638849828009484
1109	6	0.34204034553408036
1109	7	0.4146816016773869
1109	8	0.31308084436425787
1109	9	0.43974627062791427
1109	10	0.526417297055815
1109	11	0.43615438373156934
1109	12	0.42509779762286826
1109	13	0.5513727036966642
1109	14	0.5927688322945031
1109	15	0.5468475626227729
1109	16	0.41506984368250244
1109	17	0.37752563863053723
1109	18	0.44227227002011205
1109	19	0.4984414903540042
1109	20	0.3226189937779296
1109	21	0.3418720080083233
1109	22	0.2958228018163638
1109	23	0.28958158635829534
1109	24	0.4293435100238554
1109	25	0.3443864545071378
1109	26	0.4729440633763602
1109	27	0.4039954380661115
1109	28	0.3820903146228858
1109	29	0.37808189561365424
1109	30	0.5344400529530233
1109	31	0.4258164040258876
1109	32	0.5204118333111012
1109	33	0.49758308700970844
1109	34	0.5523120773344559
1109	35	0.7109784501270298
1109	36	0.7086542038444748
1109	37	0.7880140878791551
1109	38	0.7596162896803236
1109	39	0.7935114334724673
1109	40	0.8014391619222607
1109	41	0.8372862009146687
1109	42	0.7193480019396006
1109	43	0.7121867317300143
1109	44	0.7287436711064146
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1109	46	0.5518171497209821
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2216	1	-1.5316521972528783
2216	2	-1.576202263376885
2216	3	-1.385370382927917
2216	4	-1.3272520275464217
2216	5	-1.3466674401712027
2216	6	-1.5066108339205329
2216	7	-1.551867695326024
2216	8	-1.5359593183945464
2216	9	-1.870658800113766
2216	10	-1.8913956722338128
2216	11	-1.7017770719197998
2216	12	-2.1309828000899884
2216	13	-1.865361720976793
2216	14	-3.8168921217614944
2216	15	-2.8353216707055537
2216	16	-2.3868340109047423
2216	17	-2.222105850870747
2216	18	-2.5979921548743476
2216	19	-2.3174298194518883
2216	20	-2.2492989474953617
2216	21	-2.283239516436325
2216	22	-2.2491079105325795
2216	23	-2.3988632597874044
2216	24	-2.240982786945821
2216	25	-2.342642743668449
2216	26	-2.1590184062488618
2216	27	-2.4158144440175064
2216	28	-2.3819674652176843
2216	29	-2.464313794860793
2216	30	-2.377346001576761
2216	31	-2.402518773023168
2216	32	-2.49791295611562
2216	33	-2.219807049053024
2216	34	-2.2648006289494163
2216	35	-2.2186533767102574
2216	36	-2.507986929976717
2216	37	-2.4726668422841485
2216	38	-2.480924983379211
2216	39	-2.299424401730761
2216	40	-2.3789875564847662
2216	41	-2.558512686341821
2216	42	-2.3587158522754095
2216	43	-2.208296659124919
2216	44	-2.3276806603576077
2216	45	-2.316253528038402
2216	46	-2.454644579509882
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74450.0
3	2279.0
4	7439.0
5	5411.0
6	4347.0
7	3459.0
8	3071.0
9	2641.0
10	2472.0
11	2402.0
12	2400.0
13	2449.0
14	2532.0
15	2673.0
16	2836.0
17	3044.0
18	3401.0
19	4424.0
20	6410.0
21	9239.0
22	12776.0
23	14515.0
24	14602.0
25	14383.0
26	14679.0
27	17062.0
28	19204.0
29	20560.0
30	23276.0
31	27436.0
32	33853.0
33	42199.0
34	54539.0
35	73532.0
36	100244.0
37	144479.0
38	259711.0
39	777392.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.51237032096357	19.551102306376023	14.232367577779769	22.704159794880642
2	14.642815329594228	21.746249665888364	40.404258515152655	23.206676489364757
3	17.57943455520515	28.63073770718156	29.583620989611237	24.206206748002053
4	11.628162555039074	18.15813147967234	40.43807044449317	29.775635520795422
5	12.486416824584886	39.940443849582344	34.58856230370655	12.98457702212622
6	28.597747790758582	39.38209127722882	19.30378332075851	12.716377611254092
7	26.7349258011691	33.66353519470191	23.116577189468497	16.484961814660497
8	25.791951853963496	34.415706628855716	21.54931419825689	18.24302731892389
9	27.578331413533675	16.15424481778277	20.073119805985247	36.19430396269831
10	14.34705746318207	30.075156431016087	34.49468794102729	21.083098164774555
11	31.96667882754422	25.653472390484488	24.849529837660565	17.530318944310725
12	22.482243003033965	27.516735924796105	30.458913987640056	19.54210708452987
13	26.944493964911544	24.17794031529605	26.725322203462703	22.152243516329705
14	22.128565680605313	23.173591651713938	28.64306131786749	26.054781349813254
15	23.135839577971552	30.619967425038126	24.462405502530327	21.781787494459994
16	22.384992777984138	29.661373833287065	26.739175669119632	21.214457719609168
17	20.57401003965637	29.8216429381587	27.194939880727336	22.4094071414576
18	20.421998823273572	29.58390688627811	28.886205255971113	21.1078890344772
19	22.370618617584366	28.509273026922187	28.882821305056698	20.237287050436745
20	22.53280758769296	28.35278754106468	28.85489155392278	20.25951331731958
21	23.49347050966453	27.908962456810432	27.983750786519334	20.613816247005705
22	22.480587210325965	28.076835404822	28.09212389082586	21.350453494026176
23	22.37342692619958	28.2787629448637	27.830704943484974	21.517105185451747
24	21.61305919157175	28.380538378154025	28.37408075451537	21.632321675758853
25	22.27370545129205	28.128627244924825	28.420655819680775	21.177011484102355
26	21.451169218813472	28.30868392235822	28.88286176018651	21.3572850986418
27	21.751544832863246	28.746230044701193	27.79341059015845	21.70881453227711
28	21.6054782816511	27.847747748244988	28.783607985168413	21.763165984935497
29	21.894378141552917	28.320021547596507	28.516014158272174	21.2695861525784
30	22.184402251904185	28.43978364057843	28.13776355006071	21.238050557456674
31	22.521078532020162	27.69566772418087	28.473459484401115	21.309794259397858
32	21.73935840812979	28.474737361505593	28.423131394862367	21.362772835502255
33	21.925388703988872	28.078695654573654	28.13488318182571	21.861032459611764
34	22.135088518165887	28.03002580342482	28.73027831822384	21.104607360185454
35	22.13955377806135	28.454527541562513	27.93476503058579	21.471153649790345
36	21.799595856981956	28.352509882732274	28.063315739493383	21.784578520792387
37	22.500847282678894	27.79065107740134	27.95508463443202	21.753417005487744
38	21.646351224391335	28.301098369723015	28.717637616375413	21.334912789510234
39	22.10325254746347	28.618066296725587	27.72514949591892	21.553531659892023
40	22.140038277268967	27.876881225093413	28.90701118784843	21.07606930978919
41	22.100578873598792	28.19587156317962	28.0756456688304	21.62790389439119
42	22.07128845414662	28.102343366866805	27.965878917208993	21.860489261777584
43	21.864351758816163	27.687028109700485	28.350572948526278	22.098047182957075
44	21.61663340317287	28.555646650600814	28.103594389649395	21.724125556576922
45	22.499017295597483	27.815779361882555	27.747210886156452	21.93799245636351
46	22.105336960794272	27.747371047738508	27.78342114909836	22.36387084236886
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2535.0
1	2054.5
2	1574.0
3	1771.0
4	1968.0
5	1852.0
6	1736.0
7	1736.0
8	1513.0
9	1290.0
10	1398.5
11	1507.0
12	2035.0
13	2563.0
14	4479.5
15	6396.0
16	8725.5
17	11055.0
18	10945.5
19	10836.0
20	10836.0
21	11888.0
22	12940.0
23	15446.0
24	17952.0
25	26209.0
26	34466.0
27	40887.0
28	47308.0
29	55641.5
30	63975.0
31	63975.0
32	74667.0
33	85359.0
34	97787.0
35	110215.0
36	114637.5
37	119060.0
38	126023.5
39	132987.0
40	136263.5
41	139540.0
42	139414.0
43	139288.0
44	139288.0
45	135245.5
46	131203.0
47	131018.5
48	130834.0
49	128059.5
50	125285.0
51	118785.5
52	112286.0
53	103833.0
54	95380.0
55	87042.0
56	78704.0
57	78704.0
58	71129.5
59	63555.0
60	55221.0
61	46887.0
62	39076.5
63	31266.0
64	26122.5
65	20979.0
66	17242.0
67	13505.0
68	10669.0
69	7833.0
70	7833.0
71	6127.0
72	4421.0
73	3380.0
74	2339.0
75	1826.0
76	1313.0
77	980.5
78	648.0
79	522.5
80	397.0
81	397.0
82	297.0
83	197.0
84	154.0
85	111.0
86	84.5
87	58.0
88	39.5
89	21.0
90	17.0
91	13.0
92	8.0
93	3.0
94	3.0
95	4.5
96	6.0
97	6.0
98	6.0
99	13.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20438001325738028
2	0.057897551689708865
3	0.06683883231290509
4	0.05861506186317523
5	0.04178116933184901
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	3.3115854159986003E-4
18	8.278963539996501E-4
19	3.863516318665034E-4
20	0.00248368906199895
21	0.002262916700932377
22	0.0
23	1.1038618053328668E-4
24	4.9673781239979E-4
25	6.623170831997201E-4
26	7.175101734663634E-4
27	0.025388821522655935
28	6.623170831997201E-4
29	9.9347562479958E-4
30	0.0011590548955995102
31	0.0013798272566660835
32	8.278963539996501E-4
33	0.0017109857982659435
34	0.0025388821522655935
35	0.001766178888532587
36	0.03206718544491978
37	0.00811338426919657
38	0.012528831490528039
39	0.04978416742051229
40	0.01666831326052629
41	0.058670254953441865
42	0.060326047661441166
43	0.026989421140388596
44	0.029031565480254398
45	0.027210193501455163
46	0.025002469890789435
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1811821.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.95300688899948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.03303075008024	37.72205525822349
2	13.455162501789852	13.711619752926119
3	5.264884386806842	8.047850712922646
4	2.5596491125045064	5.216872754514541
5	1.4020765007771785	3.572000680150193
6	0.8364234970450508	2.5570975324234513
7	0.5419206540857446	1.9328740774645434
8	0.34587319935986016	1.40986236077626
9	0.26340161742249013	1.2078993984391573
>10	1.0984284557494433	10.048472533811386
>50	0.10998798183694067	3.964510850224391
>100	0.08380772606467271	7.891744150127095
>500	0.004151784206754338	1.4430213148011675
>1k	0.0010925747912511417	0.9287421239469108
>5k	1.0925747912511415E-4	0.3453764992487343
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6204	0.34241793201425524	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2857	0.15768665889180003	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2791	0.15404391493420155	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2181	0.12037612987154912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	5.519309026664334E-5	0.0
19	0.0	0.0	0.0	5.519309026664334E-5	0.0
20	0.0	0.0	0.0	5.519309026664334E-5	0.0
21	0.0	0.0	0.0	1.6557927079993001E-4	0.0
22	0.0	0.0	0.0	2.759654513332167E-4	0.0
23	0.0	0.0	0.0	4.415447221331467E-4	0.0
24	0.0	0.0	0.0	7.175101734663634E-4	0.0
25	0.0	0.0	0.0	7.727032637330067E-4	0.0
26	0.0	0.0	0.0	0.0010486687150662235	0.0
27	0.0	0.0	0.0	0.0012694410761327967	0.0
28	0.0	0.0	0.0	0.002097337430132447	0.0
29	0.0	0.0	0.0	0.003421971596531887	0.0
30	0.0	0.0	0.0	0.00629201229039734	0.0
31	0.0	0.0	0.0	0.008720508262129648	0.0
32	0.0	0.0	0.0	0.012859990032127898	0.0
33	0.0	0.0	0.0	0.01815852669772566	0.0
34	0.0	0.0	0.0	0.02516804916158936	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1920	0.0	27.656523	1
GTATCAA	4855	0.0	23.401667	1
TAGGGCG	140	1.8189894E-12	21.429502	31
AGTATCG	110	1.573244E-8	20.003078	5
TGGTCGG	275	0.0	18.902086	10
GGTCGGT	280	0.0	18.564548	11
TAGTCGG	270	0.0	18.525972	40
AACGCGG	65	9.34266E-4	18.46948	35
CGCTTAC	65	9.3913014E-4	18.455208	18
TTAGTCG	280	0.0	17.865318	39
TATCAAC	6445	0.0	17.424032	2
ATCAACG	6505	0.0	17.041319	3
TCAACGC	6550	0.0	16.951513	4
GTCGGTT	310	0.0	16.767979	12
CCCTATA	350	0.0	16.585886	2
CAACGCA	6690	0.0	16.564583	5
GCGTTTA	195	1.0913936E-11	16.417316	35
CTGGTCG	320	0.0	16.24398	9
CCTATAC	385	0.0	16.111713	3
AACGCAG	6880	0.0	16.040565	6
>>END_MODULE