FastQCFastQC Report
Mon 6 Feb 2017
SRR2077352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811821
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24630.13594058132674253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26250.046.628551
GTATCAA61550.038.2514841
TATATCG1750.027.58090466
CGTTCGA658.691297E-426.52156345
ATCAACG88750.026.3182543
TATCAAC89150.026.2412242
TCAACGC89700.026.077434
CAACGCA92100.025.471735
AACGCAG95550.024.768256
TGTCGTA4600.022.48691659
CGTAGTA4400.021.15814262
TTGTCGT4900.021.11016558
ACGCAGA111100.021.0902567
GTCGGAA3950.020.9475267
CGCAGAG112850.020.5672328
GAGTACT64300.020.54405412
TATGCCG2900.020.21349143
TAGGGCG2051.7535058E-920.18504731
TAGTCGG3450.019.98947140
AGAGTAC113750.019.70880311
Produced by FastQC (version 0.11.5)