FastQCFastQC Report
Mon 6 Feb 2017
SRR2077351_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077351_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2636450
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46430.1761080240474881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.023.2553311
GTATCAA34600.021.4985561
TATCAAC43500.016.8439162
ATCAACG43750.016.281113
TCAACGC43800.016.2137684
CAACGCA44350.016.055675
AACGCAG45650.015.7230286
CGGCACG1201.5850545E-514.99803722
CAACGCG850.005630164414.11285514
CGCACGA900.00818963413.33614335
ACGCAGA54000.013.1807097
CGCAGAG54550.012.8645598
AACCCGT1406.777725E-512.8527786
GTACATG65100.012.7814051
GTATAAG5650.012.7751361
GTATTAT6750.012.7725081
TACATGG63350.012.70371152
TTATACT7300.012.6056344
GTGTAGA7700.012.4986191
GTGGTAT6500.012.3383791
Produced by FastQC (version 0.11.5)