FastQCFastQC Report
Mon 6 Feb 2017
SRR2077351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2636450
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.040.7596171
GTATCAA50250.037.824681
TCAACGC65350.028.3255214
TATCAAC67000.028.1474762
ATCAACG66450.028.0136593
CAACGCA67450.027.5437895
AACGCAG70150.026.383336
ACGCAGA81150.022.450197
CGCAGAG83700.021.7546638
AGAGTAC84950.020.3392411
GAGTACT56850.020.26172412
CAGAGTA90700.019.3536410
GCAGAGT93200.019.3525379
GTACATG65000.019.156311
AGTACTT61750.018.87731213
GTACTTT62500.018.42761814
TACATGG67600.018.1027032
GCGATAC1402.091814E-417.24140714
ACATGGG69900.017.1093083
TACTTTT70200.016.30811115
Produced by FastQC (version 0.11.5)