FastQCFastQC Report
Mon 6 Feb 2017
SRR2077350_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077350_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978062
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40990.2072230294095938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25750.024.833171
GGTATCA10800.022.2728711
CGTGCCG653.800992E-521.52916310
CAGCGTA902.2802436E-517.77639622
TATCAAC35400.017.6471672
GTATAGA4100.017.1120851
ATCAACG36850.016.9959223
TCAACGC36850.016.8826224
CAACGCA37350.016.6545125
AACGCAG38850.015.9519286
TATACCG1151.0570151E-515.6533315
GGGCGTA800.00376599514.9935267
AGCGTAC956.9112837E-414.73569723
ATACCGT1151.8568117E-413.9070386
ACGCAGA44800.013.8779367
CGCAGAG44850.013.5504478
GTACATG51400.013.5327211
TAGGGTG3000.013.3343195
GAGTACT22450.013.090116512
TACATGG51050.013.021652
Produced by FastQC (version 0.11.5)