FastQCFastQC Report
Mon 6 Feb 2017
SRR2077350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978062
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.040.8937641
GTATCAA36200.038.6131481
TATCAAC50450.027.4210992
ATCAACG50500.027.1852153
TCAACGC51650.026.579934
CAACGCA52250.026.2740385
AACGCAG55800.024.8497286
ACGCAGA65000.020.9179137
TATACGC1004.3536793E-420.68775240
CGCAGAG67750.020.0052578
AGAGTAC66400.019.0110811
GAGTACT41900.018.93248612
GTACATG52200.018.7377451
TACACCG2053.6206984E-818.6018755
GTATATA7650.018.1358241
AGTACTT44250.018.00497413
TACATGG52300.017.8992652
CAGAGTA72750.017.77747710
GCAGAGT75150.017.7141069
GTACTTT46200.017.31573914
Produced by FastQC (version 0.11.5)