FastQCFastQC Report
Mon 6 Feb 2017
SRR2077349_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077349_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146088
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55650.25930903113013076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25840.12040512784191515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24800.11555910102474828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35200.023.5199151
GGTATCA14950.022.660731
CGTTACG500.00448743119.99791324
TATCAAC46300.017.8113082
TCAACGC46800.017.6489664
ATCAACG46850.017.5488453
TAGTCGG801.8621341E-417.50551840
CAACGCA47600.017.3099275
AACGCAG49100.017.0211326
TTAGTCG904.569603E-415.56118639
GAGTACT28400.014.29128612
ACGCAGA57550.014.2092657
CGCAGAG58150.013.7875878
TATACTG5150.013.2031215
AGTACTT31250.013.1798613
AGAGTAC56200.013.09197411
GTACTTT30950.012.92001314
CAGAGTA59550.012.69122910
CTGGTCG2051.0246549E-712.6789459
GCAGAGT61500.012.4838859
Produced by FastQC (version 0.11.5)