FastQCFastQC Report
Mon 6 Feb 2017
SRR2077349_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077349_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146088
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.042.999491
GTATCAA48300.038.377851
TCAACGC66150.027.906064
ATCAACG67300.027.3777483
TATCAAC67200.027.3688722
CAACGCA67900.027.1868325
AACGCAG71650.025.9089476
ACGCAGA83900.021.9191197
CGCAGAG85500.021.418248
AGAGTAC86850.020.2939811
GAGTACT57300.020.10517912
ATACCGT1901.5308615E-820.0511956
GTACATG63100.019.6574731
CAGAGTA92000.019.45607210
AGTACTT60800.019.0045413
GCAGAGT95150.018.9927589
TACATGG64150.018.681472
GTACTTT61450.018.63251914
CGTAATA1150.001081644618.071122
ACATGGG65550.017.73
Produced by FastQC (version 0.11.5)