FastQCFastQC Report
Mon 6 Feb 2017
SRR2077348_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077348_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343162
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33320.1422010087224016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29350.024.107221
GGTATCA12750.022.1661071
CTAACGT550.00776662318.1862534
TCAACGC38800.017.7878424
TATCAAC39400.017.7295192
ATCAACG39300.017.6146953
CAACGCA40000.017.2519975
TGCGATA953.634359E-516.83722713
AACGCAG42100.016.3848026
CGTTACG750.00244486315.998095524
TCGTTAC800.003758444714.99821623
ACGCAGA48550.014.2492197
GTATTAG3300.013.9699361
TTATACC4200.013.8128914
GTGCGAT1456.164595E-613.78910712
TATACTG4850.013.6098245
CGCAGAG50100.013.5290148
ATTATAC5450.012.8488163
GTCCTAA3750.012.8280471
TATCGTA1253.7564166E-412.79629212
Produced by FastQC (version 0.11.5)