FastQCFastQC Report
Mon 6 Feb 2017
SRR2077348_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077348_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343162
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37250.039.5498431
GGTATCA16950.034.9710351
TCAACGC52700.027.8103794
TATCAAC52800.027.7594932
CAACGCA53600.027.4703435
ATCAACG53550.027.3042413
AACGCAG56050.026.3920766
ACGCAGA66900.021.9156157
CGCAGAG69450.021.0536258
CGTATTA1004.3479414E-420.69202828
AGAGTAC67350.020.1240811
GTACATG61900.019.7121521
CGAACCG1258.776185E-519.31132152
GAGTACT40300.019.25478212
TACATGG63800.019.2259432
GCAGAGT76700.018.524419
CAGAGTA74250.018.4858110
ACATGGG67650.017.9769693
GTATAGG6200.017.8912371
TAATCCG1200.001436484917.3224745
Produced by FastQC (version 0.11.5)