FastQCFastQC Report
Mon 6 Feb 2017
SRR2077347_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077347_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950346
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50710.26000514780454337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24430.12525982569246688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22850.11715869902058404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.023.7539041
GTATCAA33400.022.2673841
TATAACG754.67869E-621.3567352
CGTTACG706.705352E-519.99934424
GTATACC1052.10508E-719.0582393
TATCAAC43800.016.6392382
TCAACGC44450.016.383724
ATCAACG44650.016.2688643
CAACGCA45100.016.1873845
TATCGCA1251.3005556E-616.0175532
AACGCAG46750.015.5225356
TTACACG850.00563333114.11139510
GTCCTAG3600.013.9212841
ATACCGT1456.1730952E-613.7869956
ACGCAGA54250.013.3765627
TCCTAGG3300.013.347962
CGTACAC1802.880388E-713.3407683
GTTACGG1050.001472123813.33289825
GTATTAG2751.4551915E-1113.1214421
GATACCG1406.750031E-512.8580415
Produced by FastQC (version 0.11.5)