FastQCFastQC Report
Mon 6 Feb 2017
SRR2077347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950346
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.037.8925321
GTATCAA41050.035.475941
TATCAAC57050.025.4532622
TCAACGC57350.025.0745184
ATCAACG57550.025.0482373
CAACGCA58050.024.7708785
AACGCAG60950.023.760386
ACGCAGA69900.020.4896977
AGAGTAC68950.019.91088511
GAGTACT45250.019.89590512
CGCAGAG72400.019.6757348
CGTGCCG2505.0931703E-1119.31606510
AGTACTT46200.019.26280413
GTACTTT47800.018.82823414
TATAACG950.00763445818.240282
GTACATG52900.018.1560731
CAGAGTA77450.017.9924610
GCAGAGT80200.017.3755119
TACATGG55250.017.187132
TACTTTT52900.017.01303515
Produced by FastQC (version 0.11.5)