FastQCFastQC Report
Mon 6 Feb 2017
SRR2077346_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077346_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405338
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60870.2530621476066981No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28080.11674035000486419No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27590.1147032142675998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.023.850271
GTATCAA36050.023.7365461
TATCAAC49250.017.1925642
TCAACGC50150.016.753474
ATCAACG50950.016.5713523
CAACGCA51350.016.4374795
AACGCAG52800.016.0169586
CGCGTGT750.002448827315.99423810
ACCGTGC3400.015.28860958
GTACGAA1352.8712002E-614.8443481
GTATAAG5100.013.7528521
ACGCAGA62000.013.6087
CGCAGAG61650.013.52310858
GCCCGTA1050.001475937113.3285317
GAGTACT30450.013.06590112
GTACTAG2201.8153514E-812.75264551
ATTATAC5650.012.7480483
AGAGTAC60750.012.50578211
GTGCTAG3850.012.4923871
TATGCCG1459.3745104E-512.41474155
Produced by FastQC (version 0.11.5)