FastQCFastQC Report
Mon 6 Feb 2017
SRR2077346_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077346_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405338
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.039.702871
GTATCAA54400.038.7442781
TATCAAC72850.028.8240782
TCAACGC73400.028.6541044
ATCAACG73550.028.547963
CAACGCA75000.027.9492515
AACGCAG77850.026.9700036
ACGCAGA89650.023.249117
CGCAGAG91150.022.8570028
AGAGTAC92100.021.4236511
GAGTACT62300.020.92959412
CAGAGTA97050.020.7930110
GCAGAGT102700.019.8170329
AGTACTT67700.019.41303313
GTACTTT68250.019.14873714
GTACATG63450.018.739821
TACATGG64050.018.0692312
ACATGGG65550.017.4960673
TACTTTT76300.017.17364915
ACTTTTT82150.016.07795516
Produced by FastQC (version 0.11.5)