FastQCFastQC Report
Mon 6 Feb 2017
SRR2077345_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077345_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784486
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46400.16663757691724793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16300.023.3680521
GTATCAA36150.023.3469561
GTATAGA4700.017.4880941
TACGCCG700.001540730717.14237230
TATCAAC48300.017.1197222
TCAACGC48550.016.8541874
ATCAACG48750.016.788063
CAACGCA49050.016.637115
AACGCAG51050.015.978716
ACGCAGA60000.013.3619697
GTACTAG2651.10958354E-1012.8605491
CGCAGAG61050.012.8374198
CTAGGAC5050.012.6797423
GTACATG70050.012.6780391
AGAGTAC59000.012.43630511
GTATAAA7500.012.2956951
TACATGG71850.012.2050942
TTATACT6100.012.1351634
GAGTACT25050.012.13146312
CAGAGTA62550.011.92226710
Produced by FastQC (version 0.11.5)