FastQCFastQC Report
Mon 6 Feb 2017
SRR2077345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784486
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22600.038.6646731
GTATCAA49300.038.2625771
ATCAACG68100.027.5833533
TATCAAC69000.027.3767222
TCAACGC69000.027.173344
CAACGCA69700.026.8989815
AACGCAG72500.025.9533846
ACGCAGA86700.021.6480797
CGCAGAG89650.020.8878658
GAGTACT56050.020.30551712
AGAGTAC90900.019.91916711
GTACATG75850.019.4749471
AGTACTT60150.019.03610813
CAGAGTA96200.018.99997910
TACATGG78000.018.574442
GCAGAGT99950.018.4593129
GTACTTT61800.018.24620814
TCGTATG2952.910383E-1117.53311740
ATACGCG1402.093487E-417.23966263
ACATGGG80100.017.2204863
Produced by FastQC (version 0.11.5)