FastQCFastQC Report
Mon 6 Feb 2017
SRR2077344_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077344_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413810
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57670.23891689901027838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26990.11181493158119321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26530.10990923063538556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.025.3060051
GTATCAA38600.022.7439731
TATCAAC48550.017.4789892
TCAACGC49200.016.9545214
ATCAACG49300.016.9229373
CAACGCA50100.016.6069245
AACGCAG50900.016.3401576
CGAATCG1151.0551679E-515.65652137
CGTGCCG1151.0614047E-515.64679210
TAACCCG800.00375530815.0001245
TCTATAC3900.014.8771593
TACCGAT1352.9417024E-614.8097217
GTACCGA2106.384653E-1014.2808036
ACGCAGA59650.013.7756477
CGCAGAG60100.013.5727028
GTACATG66250.013.4028571
GAGTACT28050.013.32874812
ATTATAC6050.013.227913
AGAGTAC58400.013.04345911
GTGCGCC2302.4956535E-913.03899411
Produced by FastQC (version 0.11.5)