FastQCFastQC Report
Mon 6 Feb 2017
SRR2077344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413810
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22050.042.4503171
GTATCAA49100.038.1274761
TCAACGC68900.026.8547884
ATCAACG69250.026.7196183
TATCAAC70900.026.3448942
CAACGCA70200.026.2565775
AACGCAG73850.025.2398226
ACGCAGA85700.021.5822857
CGCAGAG88300.020.8591188
AGAGTAC87350.020.25675411
GAGTACT56500.020.2066812
GTACATG64800.019.2063671
CAGAGTA93100.019.19090710
GCAGAGT95750.018.9119329
AGTACTT61600.018.70170213
TATACTG8350.018.6719465
TACATGG66350.018.2279762
GTACTTT63150.017.96511314
ACATGGG68650.017.060233
TACTTTT69400.016.59565515
Produced by FastQC (version 0.11.5)