FastQCFastQC Report
Mon 6 Feb 2017
SRR2077343_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077343_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298556
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57670.2508966498967177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26850.11681246834969433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26430.1149852342079114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.022.7309971
GTATCAA37950.022.7217451
TTAGTCG751.1351958E-418.67440839
GTGCCGA1256.84995E-817.59389311
TATCAAC48300.017.3673022
ATCAACG49250.017.141553
TCAACGC49300.016.917594
CAACGCA50000.016.4778675
AACGCAG51100.016.1189486
TAGTCGG956.888411E-414.742311540
CGTGCCG850.005631777514.11213510
ACGCAGA58000.013.9600647
CGCAGAG58800.013.7361318
GTATAAT6300.013.3688411
GTACATG61000.013.3469251
CGTATAC1050.001468994313.336833
TACGCTC1202.6483557E-413.3365434
TACATGG61600.012.8375412
AGAGTAC57100.012.74513811
ACATGGG63600.012.2044583
Produced by FastQC (version 0.11.5)