FastQCFastQC Report
Mon 6 Feb 2017
SRR2077343_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077343_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298556
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49000.038.0705221
GGTATCA21600.033.8712581
TATCAAC68050.027.1451822
TCAACGC68700.026.7340854
ATCAACG68500.026.7109643
CAACGCA69100.026.5781675
AACGCAG70950.026.0293946
ACGCAGA82800.022.0873747
CGCAGAG84550.021.3343128
GAGTACT55050.021.17721612
AGAGTAC81900.020.93061611
AGTACTT58950.019.8346913
CAGAGTA89350.019.5324210
GTACTTT59300.019.48072414
GCAGAGT92900.019.1568729
CGCTAGA1301.18396696E-418.57223513
GTACATG60500.018.1679761
TACTTTT66550.017.6693815
ACTTTTT71150.016.86701616
TACATGG63100.016.8068282
Produced by FastQC (version 0.11.5)