FastQCFastQC Report
Mon 6 Feb 2017
SRR2077342_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077342_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677926
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45590.2717044732604418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20510.12223423440604651No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.1216978579508274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13150.025.3106771
GTATCAA31950.023.5961
TACCGCG500.004492685719.99356317
TATTGCG550.007780398218.18035
TACCGTA550.007792440218.1754267
TATCAAC40900.018.1135372
ATCAACG42600.017.469583
TCAACGC42500.017.4134254
CAACGCA43350.017.1150655
AACGCAG44450.016.7320236
GGGTATA1251.2812161E-616.040261
CATACCG956.91081E-414.7356135
GTCCTAT2600.014.6521621
GTACCGT1404.288173E-614.2806916
ACGCAGA51300.014.1860437
AATCCCG850.00561948614.1164695
CGCAGAG53150.013.5418028
GTATAAT4150.013.527931
GTGGTAT4550.013.2199951
CCTATAC2007.361632E-813.0035663
Produced by FastQC (version 0.11.5)