FastQCFastQC Report
Mon 6 Feb 2017
SRR2077342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677926
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16500.043.3222961
GTATCAA41100.039.7655751
TATCAAC56250.028.9789182
TCAACGC56100.028.9249524
ATCAACG56750.028.5953673
CAACGCA57200.028.3661525
AACGCAG59950.027.2376296
ACGCAGA68650.023.416137
CGCAGAG70600.022.664218
GAGTACT43550.022.09242412
AGAGTAC71550.021.5921411
CAGAGTA75600.020.75472310
GCAGAGT76700.020.636919
AGTACTT47350.020.4650913
GTACATG55850.020.3788131
GTACTTT47550.020.1596414
TACATGG59350.019.0503832
ACATGGG60400.018.4282383
TACTTTT53900.017.72064415
CCTATAC3255.456968E-1217.070743
Produced by FastQC (version 0.11.5)