FastQCFastQC Report
Mon 6 Feb 2017
SRR2077341_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077341_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271869
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55790.2455687365776812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25860.11382698562285062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25810.11360690251066413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13950.025.146051
GTATCAA34650.023.313511
TCAACGC44750.017.1650144
ATCAACG45400.017.0115013
TATCAAC46250.016.9229932
CAACGCA45550.016.772395
AACGCAG47550.016.0616236
ATAGGAC3600.015.5620493
TAAGCGT956.8986113E-414.7394224
GTATTAC2650.014.37188151
TATACTG6300.013.9679315
GTATAGA4600.013.9443431
ACGCAGA55750.013.591617
GGACCGT1202.6616768E-413.3286256
CGCAGAG56850.013.2934558
GTATAAG3200.013.1545261
GTGGTAT5800.013.1329261
TAGGACT5050.012.6754864
GTATAAT5800.012.4417191
GACTGCG2101.403696E-712.3765787
Produced by FastQC (version 0.11.5)