##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077341_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2271869
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.747475756744777	31.0	31.0	34.0	25.0	34.0
2	29.7989514360203	31.0	30.0	34.0	25.0	34.0
3	29.95620434100734	31.0	31.0	34.0	25.0	34.0
4	33.34216585551368	37.0	35.0	37.0	27.0	37.0
5	33.42590791986686	37.0	35.0	37.0	27.0	37.0
6	33.44988289377601	37.0	35.0	37.0	28.0	37.0
7	33.36363232211012	37.0	35.0	37.0	27.0	37.0
8	33.31472721358494	37.0	35.0	37.0	27.0	37.0
9	34.75776200124215	39.0	35.0	39.0	27.0	39.0
10	34.68202215884807	39.0	35.0	39.0	27.0	39.0
11	34.706477354108	39.0	35.0	39.0	27.0	39.0
12	34.629323257635015	39.0	35.0	39.0	26.0	39.0
13	34.585483141853686	39.0	35.0	39.0	26.0	39.0
14	35.586660146337664	40.0	36.0	41.0	25.0	41.0
15	35.71826060393447	40.0	36.0	41.0	25.0	41.0
16	35.68825755358254	40.0	36.0	41.0	25.0	41.0
17	35.69956058205821	40.0	36.0	41.0	25.0	41.0
18	35.65516541666795	40.0	36.0	41.0	25.0	41.0
19	35.752318905711554	40.0	36.0	41.0	25.0	41.0
20	35.75340259495596	40.0	36.0	41.0	25.0	41.0
21	35.7597423090856	40.0	36.0	41.0	26.0	41.0
22	35.698357607766994	40.0	36.0	41.0	25.0	41.0
23	35.64114876341902	40.0	36.0	41.0	25.0	41.0
24	35.671608266145626	40.0	36.0	41.0	25.0	41.0
25	35.60230981627902	40.0	36.0	41.0	25.0	41.0
26	35.634631662300954	40.0	36.0	41.0	25.0	41.0
27	35.618018908660666	40.0	36.0	41.0	25.0	41.0
28	35.53819036220838	40.0	36.0	41.0	25.0	41.0
29	35.50249992407132	40.0	36.0	41.0	25.0	41.0
30	35.40410076461275	40.0	36.0	41.0	24.0	41.0
31	35.380542628118086	40.0	36.0	41.0	24.0	41.0
32	35.34937973976493	40.0	36.0	41.0	24.0	41.0
33	35.30526804142316	40.0	36.0	41.0	24.0	41.0
34	35.24539663158395	40.0	35.0	41.0	24.0	41.0
35	35.21601333527593	40.0	35.0	41.0	24.0	41.0
36	35.148608040340356	40.0	35.0	41.0	24.0	41.0
37	35.06230420856132	40.0	35.0	41.0	23.0	41.0
38	35.022803251419866	40.0	35.0	41.0	23.0	41.0
39	34.93966509512652	40.0	35.0	41.0	22.0	41.0
40	34.90808801035623	40.0	35.0	41.0	22.0	41.0
41	34.77546372612153	40.0	35.0	41.0	19.0	41.0
42	34.712160780397106	40.0	35.0	41.0	18.0	41.0
43	34.576601907944514	40.0	35.0	41.0	16.0	41.0
44	34.48210614256368	40.0	35.0	41.0	15.0	41.0
45	34.36588509284646	40.0	35.0	41.0	9.0	41.0
46	34.251380251238075	39.0	35.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.023232673027067108
1101	2	0.1637279455654017
1101	3	0.18311701014058812
1101	4	0.22887225022586932
1101	5	0.19576984207203196
1101	6	-0.011436933587411602
1101	7	0.1452727665521394
1101	8	0.2676443331326581
1101	9	0.21286378280071716
1101	10	0.21309419168917287
1101	11	0.22768020045256065
1101	12	0.36951511573155216
1101	13	0.3124163322764346
1101	14	0.4246661957472284
1101	15	0.39115422729104665
1101	16	0.35671478738922957
1101	17	0.2554655046206875
1101	18	0.19786423604813308
1101	19	0.2666682060668819
1101	20	0.1299221086526572
1101	21	0.1647243882422913
1101	22	0.27620321517336066
1101	23	0.32884991971015154
1101	24	0.23553681578982832
1101	25	0.2924091704611911
1101	26	0.26866386775196816
1101	27	0.2927466402567518
1101	28	0.18780379758030818
1101	29	0.2703438507369782
1101	30	0.35392434305418163
1101	31	0.30289799095739056
1101	32	0.337222790334188
1101	33	0.3249584718875269
1101	34	0.25220831030510027
1101	35	0.28632536076092663
1101	36	0.22370343977147655
1101	37	0.30814508213072145
1101	38	0.2121972627985329
1101	39	0.31371431805283834
1101	40	0.3237430256308258
1101	41	0.27295297489337855
1101	42	0.09624177578371729
1101	43	0.13950840066034687
1101	44	0.12985346441102052
1101	45	0.13311030337691676
1101	46	0.0902568705232909
1102	1	0.4995708590368828
1102	2	0.5267349114626576
1102	3	0.49704203056409213
1102	4	0.5651268167523824
1102	5	0.43238770585617203
1102	6	0.5825827988876782
1102	7	0.5845913007132353
1102	8	0.5236506293524599
1102	9	0.6206326487264349
1102	10	0.6289091273003677
1102	11	0.6348945300038196
1102	12	0.6522914828547002
1102	13	0.7233849421121903
1102	14	0.9875534738268286
1102	15	0.8136014179407454
1102	16	0.8371609604531756
1102	17	0.8307543329354914
1102	18	0.8149305922848811
1102	19	0.7848515688343625
1102	20	0.7607775985903729
1102	21	0.7532062121506229
1102	22	0.7170400285100698
1102	23	0.7247937691516384
1102	24	0.8069162777137535
1102	25	0.8558300920095263
1102	26	0.7795823898375218
1102	27	0.6737721266564805
1102	28	0.677031249717146
1102	29	0.7747272191740748
1102	30	0.775492282917952
1102	31	0.7237708505390259
1102	32	0.7639835836829221
1102	33	0.8128550367256651
1102	34	0.8389273988369013
1102	35	0.8917618236858686
1102	36	0.8310264308403603
1102	37	0.8914859626811023
1102	38	0.8138823906944097
1102	39	0.8394896289337481
1102	40	0.7314206806773527
1102	41	0.8999717173944859
1102	42	0.7817672811435301
1102	43	0.7811717815264174
1102	44	0.8647186250949588
1102	45	0.8107703200249716
1102	46	0.6889760341591042
1103	1	0.4397232466336014
1103	2	0.4068701446701404
1103	3	0.4159582657505396
1103	4	0.4857468868917394
1103	5	0.514631602012507
1103	6	0.47774685733306654
1103	7	0.4464253889099652
1103	8	0.44427149671118826
1103	9	0.6344404882096768
1103	10	0.5459604006412917
1103	11	0.5505564271432064
1103	12	0.618641384739135
1103	13	0.6327719255926496
1103	14	0.8121055096194993
1103	15	0.6984763860408947
1103	16	0.813264575586139
1103	17	0.7672985942448634
1103	18	0.6438993119656331
1103	19	0.6447829601354087
1103	20	0.6320898278735001
1103	21	0.64631743071903
1103	22	0.7321859564153925
1103	23	0.6955887890816754
1103	24	0.7889427012840216
1103	25	0.6772994576594158
1103	26	0.6584118662973566
1103	27	0.7413993599890389
1103	28	0.7416796285848477
1103	29	0.7985747079197765
1103	30	0.8022127731121955
1103	31	0.818226641580388
1103	32	0.7941114780432059
1103	33	0.8743295967026512
1103	34	0.9483487690461772
1103	35	0.9328464353235475
1103	36	0.8551694222324215
1103	37	0.8784242319479674
1103	38	0.8350170305049431
1103	39	0.8452928544656046
1103	40	0.8477677612910242
1103	41	0.9605091017124181
1103	42	0.9244346478278587
1103	43	0.9203699152535876
1103	44	0.7803199335622466
1103	45	0.7536711656887078
1103	46	0.7544242897944073
1104	1	0.47857460122537177
1104	2	0.5069459534673442
1104	3	0.5609547543400701
1104	4	0.5035094618307525
1104	5	0.5784799788375139
1104	6	0.5769478217981501
1104	7	0.6590127360083997
1104	8	0.6073449914827904
1104	9	0.6358359839428829
1104	10	0.7817869720170947
1104	11	0.7387553848638007
1104	12	0.6563210774859272
1104	13	0.7896813995646994
1104	14	1.0556577944080558
1104	15	0.9278324402723541
1104	16	0.8993486300505822
1104	17	0.8378636444535914
1104	18	0.74793559415712
1104	19	0.7154065193191954
1104	20	0.9070162643909114
1104	21	0.7864584591481147
1104	22	0.8648119386206403
1104	23	0.8148115073755235
1104	24	0.9214928223740557
1104	25	0.7793831445026385
1104	26	0.7872657199460988
1104	27	0.9138952412536625
1104	28	0.9280832937753871
1104	29	0.9577186841199605
1104	30	0.9788642739502436
1104	31	0.9790030269046284
1104	32	0.9518888206126448
1104	33	1.0213521874451885
1104	34	1.0428316953250416
1104	35	0.933536161095212
1104	36	0.9537173707298763
1104	37	0.9345636371235813
1104	38	1.0143983107371213
1104	39	0.9890514117301947
1104	40	0.9754755889199203
1104	41	1.1074311546781246
1104	42	1.0213686728982978
1104	43	1.0353166825498832
1104	44	0.9278353599636304
1104	45	1.0044424639583909
1104	46	1.0174964242977325
1105	1	0.11757718045592824
1105	2	0.021023727556229943
1105	3	0.24878698936499077
1105	4	0.25888843197425615
1105	5	0.4056295622673787
1105	6	0.4822171430807458
1105	7	0.27781139940806554
1105	8	0.35607149852764763
1105	9	0.5616789255555048
1105	10	0.3435796583851882
1105	11	0.4094841377739229
1105	12	0.4187063488135436
1105	13	0.5180509566597351
1105	14	0.7585682406159648
1105	15	0.4900722865353302
1105	16	0.5396242757197811
1105	17	0.41191032037168185
1105	18	0.2676676121363286
1105	19	0.4101222187772464
1105	20	0.3939605944341551
1105	21	0.5262228309626806
1105	22	0.39219259162763365
1105	23	0.4669291276591494
1105	24	0.3376906905422459
1105	25	0.4269694535278319
1105	26	0.5445293811841765
1105	27	0.546337143735613
1105	28	0.6685094485858656
1105	29	0.5775768144968438
1105	30	0.6342266611323666
1105	31	0.6854458827362464
1105	32	0.6680204251779358
1105	33	0.6733954232518258
1105	34	0.5842420113601179
1105	35	0.6779838546475432
1105	36	0.7238314621866166
1105	37	0.6684316742844274
1105	38	0.6306453818483391
1105	39	0.575339964129796
1105	40	0.4448151946617216
1105	41	0.5309396862023021
1105	42	0.5639744293696651
1105	43	0.5971639630295158
1105	44	0.6921140233223468
1105	45	0.6053182217502524
1105	46	0.6181573883819738
1106	1	0.04538025654670008
1106	2	0.06285526530575325
1106	3	0.09156764355203251
1106	4	0.2533413248436247
1106	5	0.23905523186023458
1106	6	0.10230454835295433
1106	7	0.16751515977898634
1106	8	0.25935054873495744
1106	9	0.17744526438195862
1106	10	0.2849226902661286
1106	11	0.2620770904068621
1106	12	0.21057723584180366
1106	13	0.2863258859705127
1106	14	0.6037620918156748
1106	15	0.42252474829552966
1106	16	0.5239173623346929
1106	17	0.4691944170922966
1106	18	0.4021389328096987
1106	19	0.3211826205540973
1106	20	0.2852015548777729
1106	21	0.2671909669226764
1106	22	0.3310005324896039
1106	23	0.3030540478828172
1106	24	0.2222712678058798
1106	25	0.32755178904066184
1106	26	0.23729612998700134
1106	27	0.27891268293907245
1106	28	0.4005620777178578
1106	29	0.41815999532177983
1106	30	0.42511377392659
1106	31	0.5291181721373022
1106	32	0.3982930805991387
1106	33	0.4818576737262603
1106	34	0.5141308105519968
1106	35	0.2460860021629614
1106	36	0.2640394934969734
1106	37	0.24445262111851918
1106	38	0.1365006897744081
1106	39	0.20381666944131638
1106	40	0.2768335886532469
1106	41	0.33925476783831954
1106	42	0.308760248413698
1106	43	0.29346868113906055
1106	44	0.3720610222530283
1106	45	0.2862924439574428
1106	46	0.19050942706345353
1107	1	0.358476093720661
1107	2	0.3486989164355201
1107	3	0.44049708394132736
1107	4	0.43366510020215543
1107	5	0.4018346842904208
1107	6	0.42748644882047415
1107	7	0.3400628458522448
1107	8	0.46011502063817034
1107	9	0.5388180918318639
1107	10	0.48824647392871867
1107	11	0.48859918336252406
1107	12	0.5126730148873335
1107	13	0.5089086982239834
1107	14	0.719015905455926
1107	15	0.7175419792231708
1107	16	0.6242655516340747
1107	17	0.5808593814263503
1107	18	0.6903267374649573
1107	19	0.6727769173246472
1107	20	0.6326917353250323
1107	21	0.5868463483697468
1107	22	0.45183259945355303
1107	23	0.5477112977437244
1107	24	0.6589444116405332
1107	25	0.4317489938935495
1107	26	0.47193494483133946
1107	27	0.6217518415715659
1107	28	0.6206065044469682
1107	29	0.6462889241808867
1107	30	0.5748343422565583
1107	31	0.7222744628791062
1107	32	0.616959282238355
1107	33	0.6145319136970286
1107	34	0.5776525630411413
1107	35	0.5798071351691121
1107	36	0.7107368851912526
1107	37	0.7214008785112114
1107	38	0.6517471958405281
1107	39	0.6913217971993006
1107	40	0.7426545696950697
1107	41	0.8067457786917274
1107	42	0.7084541607062036
1107	43	0.7031101117627827
1107	44	0.5256067734799501
1107	45	0.5189752371115759
1107	46	0.5811545696026741
1108	1	0.3814277785710729
1108	2	0.3656604424344323
1108	3	0.2907434360353207
1108	4	0.39057498862904083
1108	5	0.42949787719206256
1108	6	0.3112818621053748
1108	7	0.2097470184578185
1108	8	0.327491703920181
1108	9	0.3166339208432021
1108	10	0.4841631346128281
1108	11	0.3147949999689459
1108	12	0.12599400797925853
1108	13	0.2200479327538929
1108	14	0.3109246717487011
1108	15	0.3781691713461157
1108	16	0.3398237955685701
1108	17	0.2995553969124458
1108	18	0.45191107441305434
1108	19	0.38514289990990136
1108	20	0.3956855309258813
1108	21	0.33791819664427436
1108	22	0.4511631995656984
1108	23	0.4017708038365555
1108	24	0.4847858020261384
1108	25	0.4672485311345014
1108	26	0.319504667518693
1108	27	0.31802797322926324
1108	28	0.4772474040686774
1108	29	0.527089338885439
1108	30	0.20786317948631705
1108	31	0.39076467832238393
1108	32	0.5314051258471437
1108	33	0.4579426257768162
1108	34	0.5402159700860736
1108	35	0.5207825575216347
1108	36	0.4302418397205656
1108	37	0.43612107905953934
1108	38	0.3423694854985868
1108	39	0.4186375489224403
1108	40	0.38155989714803695
1108	41	0.45506716954299975
1108	42	0.5595337054625418
1108	43	0.513054710029067
1108	44	0.3069972625687356
1108	45	0.24643925929296984
1108	46	0.2500939620721496
1109	1	0.25367979084666104
1109	2	0.2521792571147188
1109	3	0.30041412768190057
1109	4	0.3086905172999437
1109	5	0.39736384896232835
1109	6	0.34290574728283474
1109	7	0.3721582056553956
1109	8	0.31851765577991387
1109	9	0.39700838159964746
1109	10	0.5047476398978645
1109	11	0.3620632184908885
1109	12	0.3983157006831064
1109	13	0.40140820918615105
1109	14	0.5545222397827771
1109	15	0.4901281270979183
1109	16	0.42158993557510627
1109	17	0.40827053097412147
1109	18	0.4121980535944587
1109	19	0.3919610992673128
1109	20	0.24669674924974316
1109	21	0.28585868499249756
1109	22	0.3023559342797384
1109	23	0.26654993585058406
1109	24	0.24720801847470852
1109	25	0.42633997809505786
1109	26	0.4036382378576633
1109	27	0.3593067939804584
1109	28	0.42423400690502433
1109	29	0.3842862627855368
1109	30	0.44812464026077237
1109	31	0.4042557353040621
1109	32	0.45525248873072144
1109	33	0.4211464077487861
1109	34	0.4834728853942849
1109	35	0.4764460939818349
1109	36	0.2933681918488631
1109	37	0.5016205208443836
1109	38	0.44136601659014474
1109	39	0.5182358655307553
1109	40	0.4221481838905561
1109	41	0.5151582589371699
1109	42	0.45420596628535037
1109	43	0.5319706793555312
1109	44	0.4541467639051078
1109	45	0.5210069962578743
1109	46	0.3736567831423301
1110	1	0.0730028925670716
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2216	12	-2.2989066581600994
2216	13	-2.4564168940993056
2216	14	-4.177322881208763
2216	15	-3.005259685217645
2216	16	-2.6416638023749286
2216	17	-2.6181754654846685
2216	18	-2.7268463099703695
2216	19	-2.5963836282558077
2216	20	-2.517200063656027
2216	21	-2.5736964497704378
2216	22	-2.6454787597985643
2216	23	-2.641780528442162
2216	24	-2.5879520739903725
2216	25	-2.582173694932685
2216	26	-2.5203141524630155
2216	27	-2.6456521377527693
2216	28	-2.6070184522726265
2216	29	-2.9390968279089407
2216	30	-2.6643974923380895
2216	31	-2.710230050649116
2216	32	-2.685301809911813
2216	33	-2.5773842336047466
2216	34	-2.5211405318789772
2216	35	-2.477305381524758
2216	36	-2.6993415760047696
2216	37	-2.5360380961141047
2216	38	-2.5466344260618357
2216	39	-2.668966573973478
2216	40	-2.6921679188047065
2216	41	-2.7138624965222036
2216	42	-2.690724438987125
2216	43	-2.642113276969475
2216	44	-2.6625990063093354
2216	45	-2.707679311040966
2216	46	-2.845214946151394
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	98518.0
3	3006.0
4	9233.0
5	6992.0
6	5701.0
7	4620.0
8	3876.0
9	3443.0
10	3213.0
11	3109.0
12	3142.0
13	3255.0
14	3267.0
15	3509.0
16	3807.0
17	4127.0
18	4474.0
19	5465.0
20	7541.0
21	10157.0
22	13002.0
23	14562.0
24	15740.0
25	16271.0
26	17999.0
27	20748.0
28	23429.0
29	25587.0
30	28975.0
31	34956.0
32	42913.0
33	53143.0
34	68404.0
35	91957.0
36	127209.0
37	185736.0
38	338869.0
39	961914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.83962584126394	18.246148567023027	13.73226779483547	24.18195779687757
2	15.3010229720582	20.356734364188974	39.07711862503254	25.265124038720288
3	18.030226416224586	25.625371091100334	29.7616824323491	26.582720060325975
4	12.56417495136643	17.020053722642654	38.252168746705095	32.16360257928582
5	13.848478216547383	37.667448581434584	34.60204031756279	13.882032884455251
6	31.223015059407032	37.363818072256805	17.990782038929183	13.422384829406978
7	27.726070473253518	32.25533690542897	22.295827796409036	17.72276482490848
8	26.412966592704066	34.19818660318883	20.44475275643094	18.944094047676163
9	26.71553685533805	15.882870007029453	19.40552030068635	37.99607283694614
10	14.654718207783986	28.79439791642916	33.91868105071199	22.632202825074863
11	33.882499387068535	23.608667577223862	24.101477682031845	18.40735535367576
12	23.38721994974182	24.924720571476612	30.37789590861093	21.31016357017064
13	27.58059553609825	22.470397720995354	26.170302953207248	23.778703789699144
14	22.463399078027827	21.420865375600442	27.221111780652844	28.894623765718887
15	23.44733360154164	28.998541287206198	23.998774575613176	23.555350535638986
16	23.370405599970773	28.403398259318653	25.353046324413953	22.873149816296625
17	22.348149553427	28.05361428998392	26.62852459463778	22.969711561951303
18	22.312912054778362	27.925440368757386	27.434826128179463	22.326821448284786
19	23.340418592224058	27.383285802382463	27.474488886384812	21.801806719008667
20	23.499886214126086	26.87926064272072	27.501187378703595	22.119665764449596
21	23.713797493093594	26.661675038867788	27.303675845275382	22.320851622763232
22	23.491715411407966	26.43189374035211	27.236473581883462	22.839917266356466
23	23.377012668034123	26.56902273907723	27.067072794978564	22.986891797910083
24	22.97429586082192	26.74153069830253	27.18037970783308	23.10379373304247
25	23.2919396825313	26.544889046176547	27.193260458675457	22.9699108126167
26	23.039224101313337	26.84124739253549	27.229742548230668	22.88978595792051
27	23.118170446975657	26.926275719448928	27.039823677060625	22.915730156514787
28	23.073967161506545	26.767879512959947	27.15148826108812	23.00666506444539
29	23.198231211130025	26.8240635221629	27.089003304374387	22.888701962332686
30	23.34460909353012	26.804059273670845	27.065565941188513	22.78576569161053
31	23.384676873750177	26.44200401352043	27.10460023038787	23.06871888234152
32	23.172551976523025	26.79208026258857	27.022730003789874	23.01263775709853
33	23.33020074257807	26.636984085411886	26.930493900251562	23.102321271758484
34	23.386311905234574	26.607107406949293	27.104642620231807	22.901938067584325
35	23.3608444368147	26.775522600921732	26.934822894520227	22.928810067743342
36	23.378930733839724	26.740416245983788	26.863219686945893	23.017433333230596
37	23.43206617915068	26.48583497824491	27.028259610186772	23.05383923241764
38	23.336450136866414	26.616621719542273	27.033516203416337	23.013411940174983
39	23.42612887442633	26.886485215293494	26.820337949989675	22.867047960290503
40	23.451664823515326	26.602000817091298	27.076715011235002	22.869619348158373
41	23.282483765604574	26.719233926803398	26.949668969842605	23.04861333774942
42	23.39058298630457	26.639697154547637	26.933777882504245	23.03594197664355
43	23.28408980336197	26.475049411574087	27.012643815308806	23.22821696975514
44	23.347927815433227	26.71948213658287	26.8228205803332	23.109769467650707
45	23.309921601813294	26.767173324010905	26.700293145640288	23.222611928535514
46	23.454092444274593	26.539193253469094	26.672686790788063	23.334027511468246
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	741.0
1	726.5
2	712.0
3	853.0
4	994.0
5	1005.5
6	1017.0
7	1017.0
8	983.5
9	950.0
10	1126.5
11	1303.0
12	1844.0
13	2385.0
14	4472.5
15	6560.0
16	9077.5
17	11595.0
18	12288.5
19	12982.0
20	12982.0
21	14547.5
22	16113.0
23	20139.0
24	24165.0
25	30144.5
26	36124.0
27	42245.5
28	48367.0
29	55416.5
30	62466.0
31	62466.0
32	69606.0
33	76746.0
34	85612.0
35	94478.0
36	103208.5
37	111939.0
38	119393.0
39	126847.0
40	135063.5
41	143280.0
42	148518.0
43	153756.0
44	153756.0
45	159191.5
46	164627.0
47	167730.0
48	170833.0
49	172330.0
50	173827.0
51	170140.0
52	166453.0
53	160687.0
54	154921.0
55	145352.0
56	135783.0
57	135783.0
58	125311.0
59	114839.0
60	101373.5
61	87908.0
62	75642.5
63	63377.0
64	53403.0
65	43429.0
66	35049.0
67	26669.0
68	21179.0
69	15689.0
70	15689.0
71	12339.0
72	8989.0
73	7003.5
74	5018.0
75	3917.5
76	2817.0
77	2175.0
78	1533.0
79	1142.0
80	751.0
81	751.0
82	592.0
83	433.0
84	305.0
85	177.0
86	142.5
87	108.0
88	77.0
89	46.0
90	37.0
91	28.0
92	24.5
93	21.0
94	21.0
95	24.5
96	28.0
97	24.0
98	20.0
99	22.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20230039672181804
2	0.05845407459673071
3	0.06765354868612583
4	0.05801390837235774
5	0.041727758070557765
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.3204986731189168E-4
16	0.0
17	5.281994692475667E-4
18	0.001012382316057836
19	7.042659589967556E-4
20	0.002552964101363239
21	0.002861080458424319
22	0.0
23	3.96149601935675E-4
24	7.482825814340527E-4
25	0.001012382316057836
26	0.0011444321833697277
27	0.025617674258506983
28	0.001012382316057836
29	0.0014085319179935113
30	0.0014965651628681053
31	0.002112797876990267
32	0.0011444321833697277
33	0.00237689761161405
34	0.0030811635706108056
35	0.002596980723800536
36	0.03345263305234589
37	0.009551607068893497
38	0.013689169577999434
39	0.05185158123113613
40	0.017474599107606997
41	0.060786955585907465
42	0.0617993379019653
43	0.028170638359870222
44	0.03041548610417238
45	0.02830268822718211
46	0.02632194021750374
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2271869.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.46883870201623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.20142586518733	54.78565904072054
2	11.28569023571534	15.228648283087958
3	3.3669620755042344	6.814950635640031
4	1.43694092147379	3.877949410209669
5	0.725567461968395	2.4476597029488474
6	0.4912526935750869	1.9886549246849123
7	0.3182852142512871	1.5032033647089735
8	0.2296552218157478	1.2395656894209957
9	0.15448561212133408	0.938066836139688
>10	0.7561637452530616	8.724074940015969
>50	0.025822063802235225	1.1650533942161896
>100	0.0074868371044060685	0.7864248101517785
>500	6.551305693822759E-5	0.02510611046978649
>1k	1.3102611387632418E-4	0.2283860436571428
>5k	6.551305693816209E-5	0.2465968139274627
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5579	0.2455687365776812	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2586	0.11382698562285062	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2581	0.11360690251066413	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.401662243729722E-5
2	0.0	0.0	0.0	0.0	4.401662243729722E-5
3	0.0	0.0	0.0	0.0	4.401662243729722E-5
4	0.0	0.0	0.0	0.0	4.401662243729722E-5
5	0.0	0.0	0.0	0.0	4.401662243729722E-5
6	0.0	0.0	0.0	0.0	4.401662243729722E-5
7	0.0	0.0	0.0	0.0	4.401662243729722E-5
8	0.0	0.0	0.0	0.0	4.401662243729722E-5
9	0.0	0.0	0.0	0.0	8.803324487459444E-5
10	0.0	0.0	0.0	0.0	8.803324487459444E-5
11	0.0	0.0	0.0	0.0	8.803324487459444E-5
12	0.0	0.0	0.0	0.0	1.3204986731189165E-4
13	0.0	4.401662243729722E-5	0.0	0.0	1.3204986731189165E-4
14	0.0	4.401662243729722E-5	0.0	0.0	1.3204986731189165E-4
15	4.401662243729722E-5	4.401662243729722E-5	0.0	0.0	1.3204986731189165E-4
16	4.401662243729722E-5	4.401662243729722E-5	0.0	0.0	1.3204986731189165E-4
17	4.401662243729722E-5	4.401662243729722E-5	0.0	0.0	1.3204986731189165E-4
18	4.401662243729722E-5	4.401662243729722E-5	0.0	0.0	1.3204986731189165E-4
19	8.803324487459444E-5	4.401662243729722E-5	0.0	0.0	1.3204986731189165E-4
20	8.803324487459444E-5	4.401662243729722E-5	0.0	0.0	1.3204986731189165E-4
21	1.3204986731189165E-4	4.401662243729722E-5	0.0	4.401662243729722E-5	1.3204986731189165E-4
22	1.7606648974918888E-4	4.401662243729722E-5	0.0	4.401662243729722E-5	1.3204986731189165E-4
23	1.7606648974918888E-4	4.401662243729722E-5	0.0	4.401662243729722E-5	2.200831121864861E-4
24	2.640997346237833E-4	4.401662243729722E-5	0.0	8.803324487459444E-5	2.200831121864861E-4
25	3.0811635706108056E-4	4.401662243729722E-5	0.0	8.803324487459444E-5	2.200831121864861E-4
26	3.5213297949837776E-4	4.401662243729722E-5	0.0	1.7606648974918888E-4	2.200831121864861E-4
27	3.5213297949837776E-4	4.401662243729722E-5	0.0	3.5213297949837776E-4	2.200831121864861E-4
28	3.96149601935675E-4	4.401662243729722E-5	0.0	6.162327141221611E-4	2.200831121864861E-4
29	4.401662243729722E-4	4.401662243729722E-5	0.0	0.0013204986731189165	2.200831121864861E-4
30	4.401662243729722E-4	4.401662243729722E-5	0.0	0.0018927147648037804	2.200831121864861E-4
31	4.401662243729722E-4	4.401662243729722E-5	0.0	0.004401662243729722	2.200831121864861E-4
32	4.401662243729722E-4	4.401662243729722E-5	0.0	0.006778559855343772	2.200831121864861E-4
33	4.401662243729722E-4	4.401662243729722E-5	0.0	0.009243490711832416	2.200831121864861E-4
34	4.401662243729722E-4	8.803324487459444E-5	0.0	0.012324654282443222	2.200831121864861E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1395	0.0	25.14605	1
GTATCAA	3465	0.0	23.31351	1
TCAACGC	4475	0.0	17.165014	4
ATCAACG	4540	0.0	17.011501	3
TATCAAC	4625	0.0	16.922993	2
CAACGCA	4555	0.0	16.77239	5
AACGCAG	4755	0.0	16.061623	6
ATAGGAC	360	0.0	15.562049	3
TAAGCGT	95	6.8986113E-4	14.739422	4
GTATTAC	265	0.0	14.3718815	1
TATACTG	630	0.0	13.967931	5
GTATAGA	460	0.0	13.944343	1
ACGCAGA	5575	0.0	13.59161	7
GGACCGT	120	2.6616768E-4	13.328625	6
CGCAGAG	5685	0.0	13.293455	8
GTATAAG	320	0.0	13.154526	1
GTGGTAT	580	0.0	13.132926	1
TAGGACT	505	0.0	12.675486	4
GTATAAT	580	0.0	12.441719	1
GACTGCG	210	1.403696E-7	12.376578	7
>>END_MODULE