FastQCFastQC Report
Mon 6 Feb 2017
SRR2077341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271869
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44650.039.053851
GGTATCA20300.038.424111
TATCAAC59200.029.0338272
ATCAACG60550.028.4418453
TCAACGC60550.028.383994
CAACGCA61700.027.7987945
AACGCAG63900.026.9501676
ACGCAGA75050.022.5298737
CGCAGAG77750.021.5580838
AGAGTAC77650.020.56519711
GCGTATA1701.0932126E-720.28154867
GAGTACT50900.020.05706212
CAGAGTA82550.019.92898410
GCAGAGT85200.019.389669
GTACATG58500.019.2002321
CGCGTAT1651.8408373E-618.80652666
AGTACTT55800.018.6048313
GTATAGG5250.018.489111
TACATGG60450.018.4014952
GTACTTT57300.017.87269614
Produced by FastQC (version 0.11.5)