FastQCFastQC Report
Mon 6 Feb 2017
SRR2077340_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077340_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776908
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75460.4246702699295631No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35920.20214890134998548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34600.19472026689057623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17250.027.6513751
TCGAACG751.7671482E-723.9962525
GTATCAA41900.023.0069961
CGAACGT951.7161365E-618.94440826
CGCGTCT801.8714485E-417.49381812
TATCAAC57050.016.8057462
ATCAACG58500.016.4816253
TCAACGC59150.016.3663024
CAACGCA60350.016.1057995
AACGCAG61950.015.7486346
GCGACGA1404.288586E-614.28066813
CGACGAC1303.3771088E-513.84204314
ATAGACG1303.3797936E-513.8408758
CCCTATA1601.1131579E-613.7610212
GTATTAC2251.7316779E-913.3609581
ATAGGAC2851.8189894E-1213.3357543
CGACACG900.00820660713.33162527
ATTATAC4000.013.002363
ACGCAGA74000.012.9950437
TATACTA3550.012.9575455
Produced by FastQC (version 0.11.5)