FastQCFastQC Report
Mon 6 Feb 2017
SRR2077340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776908
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27880.15690176418812904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25300.044.5458641
GTATCAA62150.040.1731531
ATCGCGT400.002601197234.487878
TATCAAC89850.027.7393072
ATCAACG91900.027.1559433
TCAACGC91900.027.1189944
CAACGCA93400.026.6069835
AACGCAG96400.025.8141886
CCGCGAT750.001993608422.99968122
GTGGTAT8450.022.981371
ACGCAGA111450.022.1304457
CGCAGAG113150.021.732088
GAGTACT75200.021.18981412
AGTACTT77550.020.85902813
AGAGTAC113850.020.5096711
GCAGAGT119900.019.9621189
GTACTTT79800.019.91895514
CAGAGTA118200.019.84129510
GTACATG78000.018.939111
TACGTTG1108.2664605E-418.80891633
Produced by FastQC (version 0.11.5)