FastQCFastQC Report
Mon 6 Feb 2017
SRR2077339_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077339_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080916
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57470.27617645306201694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26680.1282127678387787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25050.12037967894907821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34550.023.6169051
GGTATCA13300.023.5152511
CGGGATA550.0077889718.17707828
ATCAACG44500.017.9858063
TCAACGC44900.017.8658454
TATCAAC45050.017.8645062
CAACGCA45050.017.7149935
AACGCAG46700.017.2113726
CTATGCG1058.460361E-515.2531812
ACGCAGA53700.014.8561047
CGCAGAG54800.014.4484418
GAGTACT28350.014.17619712
TAATGCG850.00561415814.1187165
AGAGTAC53050.013.605926511
AGTACTT29700.013.53182513
TATGCGC900.00817536913.3394733
ATCGATC900.00819097413.33562540
TCGCGTC1050.001474714313.32985819
CAGAGTA55600.013.30556310
GTATTAT4550.013.2186321
Produced by FastQC (version 0.11.5)