FastQCFastQC Report
Mon 6 Feb 2017
SRR2077339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080916
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.041.8448751
GTATCAA48550.040.271651
ATCAACG66800.029.2565693
TATCAAC67350.028.9147532
TCAACGC68100.028.7998434
CAACGCA69400.028.057935
GCGCGAA500.007749286527.5905413
AACGCAG72750.026.9564236
ACGCAGA82300.023.7304557
CGCAGAG85050.022.828828
AGAGTAC85900.021.27908311
CAGAGTA89400.020.87036310
GAGTACT59600.020.83178312
ATACCGC1356.579414E-620.5321776
GCAGAGT92950.020.3696129
AGTACTT63250.019.8477413
GTACTTT64700.019.24070514
GTACATG57350.018.6177351
TACATGG57500.017.7776742
TACTTTT73850.016.85678315
Produced by FastQC (version 0.11.5)