FastQCFastQC Report
Mon 6 Feb 2017
SRR2077338_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077338_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2762893
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61470.22248418596015118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28710.10391281891843078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28130.10181357005139179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.024.746321
GTATCAA39850.024.4002951
TATCAAC53500.017.9219862
TCAACGC54200.017.3070874
ATCAACG54250.017.295523
CAACGCA54850.017.1727455
AACGCAG56800.016.6138826
GTATTAT5450.016.1859361
GTATAGA5550.015.1718311
GCGATAC1201.5881422E-514.99500514
ACGCAGA65350.014.3178497
CGCAGAG66200.013.8924028
CGTGCCG1456.1694664E-613.78826110
GTCGAAT1202.646906E-413.33758435
TAACCCG1050.001472915613.3322725
CGTATTA1354.8188995E-513.32889428
AGAGTAC64500.013.23566111
GAGTACT30650.013.1764512
GTGGTAT7200.013.0872081
CAGAGTA68800.012.90244510
Produced by FastQC (version 0.11.5)