FastQCFastQC Report
Mon 6 Feb 2017
SRR2077338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2762893
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.041.5360531
GTATCAA55400.037.8793871
TATCAAC75800.027.3984012
TCAACGC76700.027.0744444
ATCAACG77200.026.9893953
CAACGCA77700.026.679925
AACGCAG81050.025.7454456
ACGCAGA93600.022.010997
CGCAGAG96400.021.4662288
GAGTACT65650.020.64653812
AGAGTAC96750.020.31951111
CAGAGTA103650.019.59906610
AGTACTT70600.019.58975813
GTACATG67350.019.1585521
GCAGAGT108400.018.9311549
GTACTTT72100.018.89074114
CGAATCG2307.3268893E-917.99006737
TACATGG70100.017.5581172
ACATGGG73050.017.3224223
TGCGCGA1250.001945420216.55505610
Produced by FastQC (version 0.11.5)