FastQCFastQC Report
Mon 6 Feb 2017
SRR2077337_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077337_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963955
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47010.23936393654640764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22590.11502300205452771No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21760.11079683597638439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29300.024.7620371
GGTATCA11550.022.5583231
ATCAACG38400.018.4439243
TCAACGC38700.018.3498424
TATCAAC38950.018.2969382
CAACGCA39700.017.7827765
AACGCAG40750.017.4165156
GTATTAG3400.015.326391
TAGTCGG956.887634E-414.742352540
ACGCAGA47400.014.5934617
GTGGTAT5650.014.5438981
CGCAGAG47650.014.5168948
GACCGTC1000.00102140513.9945387
CGAATCG1303.3537926E-513.85238837
CGTGCCG1456.169781E-613.78772210
TGGACCG1903.7773134E-813.6837495
TACCGAT1354.820103E-513.3281317
AGAGTAC49150.013.26033911
GAGTACT24000.012.99492812
CCCTATA1702.1978285E-612.9532762
Produced by FastQC (version 0.11.5)