##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077337_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1963955 Sequences flagged as poor quality 0 Sequence length 46 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.715180846811663 31.0 30.0 34.0 25.0 34.0 2 29.78295531211255 31.0 30.0 34.0 25.0 34.0 3 29.89503832827127 31.0 31.0 34.0 25.0 34.0 4 33.30766794554865 37.0 35.0 37.0 27.0 37.0 5 33.358297415164806 37.0 35.0 37.0 27.0 37.0 6 33.47219615520722 37.0 35.0 37.0 28.0 37.0 7 33.326189754856905 37.0 35.0 37.0 27.0 37.0 8 33.273998640498384 37.0 35.0 37.0 27.0 37.0 9 34.72635625561686 39.0 35.0 39.0 27.0 39.0 10 34.657401518873904 39.0 35.0 39.0 27.0 39.0 11 34.68365262951544 39.0 35.0 39.0 27.0 39.0 12 34.607242019292705 39.0 35.0 39.0 26.0 39.0 13 34.56832564900927 39.0 35.0 39.0 26.0 39.0 14 35.55482992227419 40.0 36.0 41.0 25.0 41.0 15 35.702154071758265 40.0 36.0 41.0 26.0 41.0 16 35.668577436855735 40.0 36.0 41.0 25.0 41.0 17 35.673432945255875 40.0 36.0 41.0 25.0 41.0 18 35.628911558564226 40.0 36.0 41.0 25.0 41.0 19 35.7310814147982 40.0 36.0 41.0 25.0 41.0 20 35.741839807938575 40.0 36.0 41.0 25.0 41.0 21 35.7492834611791 40.0 36.0 41.0 26.0 41.0 22 35.685041663378236 40.0 36.0 41.0 25.0 41.0 23 35.62639266174632 40.0 36.0 41.0 25.0 41.0 24 35.659858296142225 40.0 36.0 41.0 25.0 41.0 25 35.5881142897877 40.0 36.0 41.0 25.0 41.0 26 35.618613461102726 40.0 36.0 41.0 25.0 41.0 27 35.605185964036856 40.0 36.0 41.0 25.0 41.0 28 35.52767960569361 40.0 36.0 41.0 25.0 41.0 29 35.4954838578277 40.0 36.0 41.0 25.0 41.0 30 35.39621274418202 40.0 36.0 41.0 24.0 41.0 31 35.37289805519984 40.0 36.0 41.0 24.0 41.0 32 35.34364025652319 40.0 36.0 41.0 24.0 41.0 33 35.29775682233045 40.0 35.0 41.0 24.0 41.0 34 35.23859660735607 40.0 35.0 41.0 24.0 41.0 35 35.20939532728601 40.0 35.0 41.0 24.0 41.0 36 35.134898712037696 40.0 35.0 41.0 24.0 41.0 37 35.045851356064674 40.0 35.0 41.0 23.0 41.0 38 35.00896609138193 40.0 35.0 41.0 23.0 41.0 39 34.93050757272952 40.0 35.0 41.0 22.0 41.0 40 34.89784898330155 40.0 35.0 41.0 22.0 41.0 41 34.76349559944092 40.0 35.0 41.0 19.0 41.0 42 34.70583032706961 40.0 35.0 41.0 19.0 41.0 43 34.58201384451273 40.0 35.0 41.0 18.0 41.0 44 34.4900066447551 40.0 35.0 41.0 15.0 41.0 45 34.36924471283711 40.0 35.0 41.0 12.0 41.0 46 34.25149099648414 39.0 35.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0061679747818743635 1101 2 0.06848478430428173 1101 3 0.11808588692291622 1101 4 0.23927995197369967 1101 5 0.06546598727884145 1101 6 0.12417624633492608 1101 7 0.10159013455307786 1101 8 0.22886712487853345 1101 9 0.07738952343933647 1101 10 0.24655653950952683 1101 11 0.1327925131780816 1101 12 0.3130417948921149 1101 13 0.27390087929763496 1101 14 0.40689081948519856 1101 15 0.29958195610918636 1101 16 0.17298153393561222 1101 17 0.1660350285212857 1101 18 0.27653974844156437 1101 19 0.10147503126416524 1101 20 0.12525568409607502 1101 21 0.0693284190273431 1101 22 0.23268659093112376 1101 23 0.2712882148825244 1101 24 0.1744320423105279 1101 25 0.21334783048904882 1101 26 0.225242481495755 1101 27 0.24672586826621057 1101 28 0.18285664151621006 1101 29 0.19067116504344028 1101 30 0.2949639679818574 1101 31 0.11265673734791193 1101 32 0.11668840546467862 1101 33 0.2033621110926589 1101 34 0.033274355034443204 1101 35 0.09601973169070988 1101 36 -0.07862172655283928 1101 37 0.1110114886312985 1101 38 -0.0401289630564321 1101 39 0.17202812624955044 1101 40 0.06513988796631764 1101 41 0.08236251252993299 1101 42 -0.04736597212053084 1101 43 0.004734585807653957 1101 44 -0.03281653793455064 1101 45 -0.12629195251141567 1101 46 -0.1478660116376176 1102 1 0.29673338089081724 1102 2 0.2852940311424099 1102 3 0.39932115713003924 1102 4 0.5388070274236867 1102 5 0.3752612171827181 1102 6 0.44674249175697156 1102 7 0.46466806193627264 1102 8 0.4467283006669831 1102 9 0.6078304618481454 1102 10 0.5920036967418554 1102 11 0.4635415545061434 1102 12 0.6161054144197209 1102 13 0.5918683883521823 1102 14 0.9024697275875084 1102 15 0.7482253453528003 1102 16 0.7773124351531209 1102 17 0.61397696285416 1102 18 0.621872868567749 1102 19 0.6685723104908661 1102 20 0.6569618008437175 1102 21 0.6215894707434302 1102 22 0.7234186686061008 1102 23 0.7255543937069007 1102 24 0.7095123903213718 1102 25 0.6575527688532574 1102 26 0.7539951639019051 1102 27 0.678461102488285 1102 28 0.7219040870343463 1102 29 0.842382456541543 1102 30 0.7302356195736621 1102 31 0.6980704031492451 1102 32 0.6548219069309411 1102 33 0.7410360430211256 1102 34 0.7597696602534896 1102 35 0.6772108568669566 1102 36 0.6444999809424914 1102 37 0.7975863309306561 1102 38 0.6324218493439133 1102 39 0.8199561733970526 1102 40 0.7529513993279622 1102 41 0.7179729050101784 1102 42 0.7354743070017591 1102 43 0.767774095356863 1102 44 0.8212059562647624 1102 45 0.833388201281565 1102 46 0.7803572598386097 1103 1 0.37210741546052617 1103 2 0.42833277716055207 1103 3 0.4904861385769337 1103 4 0.4400501350316972 1103 5 0.47006374674631246 1103 6 0.4734172253502251 1103 7 0.509167152858879 1103 8 0.4168547045351474 1103 9 0.6807361159627732 1103 10 0.64684979761725 1103 11 0.6147688089933183 1103 12 0.5804373787700214 1103 13 0.49449591927815106 1103 14 0.7529627844159918 1103 15 0.7113833117937318 1103 16 0.7073688375845961 1103 17 0.5896367251564314 1103 18 0.49008847596520866 1103 19 0.5052772393252098 1103 20 0.5764013187106798 1103 21 0.5593221276769356 1103 22 0.440068780321063 1103 23 0.3903844928384501 1103 24 0.518719090246563 1103 25 0.4416375979120275 1103 26 0.46743613332024836 1103 27 0.4911083011922983 1103 28 0.5426041757127393 1103 29 0.6082514304978375 1103 30 0.5782756536578759 1103 31 0.6432337150643548 1103 32 0.544612868670427 1103 33 0.6210194963262552 1103 34 0.5319724513935782 1103 35 0.5274622774552071 1103 36 0.5661199221868642 1103 37 0.5904032571096067 1103 38 0.5711437162470574 1103 39 0.6133714310350342 1103 40 0.4945001996925882 1103 41 0.6463029273531689 1103 42 0.5000292058637967 1103 43 0.5294169740854429 1103 44 0.5187238281814572 1103 45 0.44276272135309824 1103 46 0.5201972671706727 1104 1 0.5417728537235185 1104 2 0.5137577189410258 1104 3 0.5559128548983345 1104 4 0.6107615800914274 1104 5 0.48354381597771834 1104 6 0.6757337305255646 1104 7 0.7098204513172135 1104 8 0.703938598996146 1104 9 0.7573171590039038 1104 10 0.7577846160549697 1104 11 0.7273533427752312 1104 12 0.6752528022046533 1104 13 0.7256897963826816 1104 14 0.9838625252844011 1104 15 0.8187689268301668 1104 16 0.8972698765418485 1104 17 0.8063390447002021 1104 18 0.7360662237081428 1104 19 0.6743175773144898 1104 20 0.7788989242958593 1104 21 0.7137905675880631 1104 22 0.8900898074156274 1104 23 0.858410911620382 1104 24 0.8735971330980803 1104 25 0.7012474663042951 1104 26 0.7501628005379146 1104 27 0.8232575133447426 1104 28 0.7632611691056184 1104 29 0.9216983182447507 1104 30 0.8963362262754941 1104 31 0.91838503721889 1104 32 0.8435985848313905 1104 33 0.8753977506641561 1104 34 0.8950797454860577 1104 35 0.8488185129003298 1104 36 0.9285139966786886 1104 37 0.8107719483735139 1104 38 0.8849376118262597 1104 39 0.7497497631610344 1104 40 0.8830947632770574 1104 41 0.9812691624579628 1104 42 1.0119512038510337 1104 43 0.8743726591797909 1104 44 0.7907904877175511 1104 45 0.9689679506578202 1104 46 0.8933634128191628 1105 1 0.014205910869844018 1105 2 0.03421173692597179 1105 3 0.28043109843849123 1105 4 0.1437959889950733 1105 5 0.22306451586614884 1105 6 0.2747523693198488 1105 7 0.1718540249446363 1105 8 0.23013764449572705 1105 9 0.2742094130833834 1105 10 0.1602204653094077 1105 11 0.2529351792283805 1105 12 0.21017347452544044 1105 13 0.2692050958392258 1105 14 0.6282007308660269 1105 15 0.4500375924263267 1105 16 0.40139253608961667 1105 17 0.29611415629617 1105 18 0.16752562445341113 1105 19 0.3416037306966473 1105 20 0.37947419281537265 1105 21 0.22477662943149568 1105 22 0.2373083611507525 1105 23 0.32370901334029867 1105 24 0.2507192825265179 1105 25 0.29058125988385797 1105 26 0.3065554911051791 1105 27 0.2833678310677925 1105 28 0.405911150668274 1105 29 0.3543385333195417 1105 30 0.5399362314591514 1105 31 0.5111590143000413 1105 32 0.5039059994688699 1105 33 0.47672300062851747 1105 34 0.46808983830892714 1105 35 0.4984712665433193 1105 36 0.5692036120392885 1105 37 0.5390873411359465 1105 38 0.5156529216501227 1105 39 0.45863173003037616 1105 40 0.2870950177476743 1105 41 0.3492832667272978 1105 42 0.4082587881355906 1105 43 0.49135515607842706 1105 44 0.49131792518669926 1105 45 0.507420463024971 1105 46 0.5058784189276366 1106 1 0.0849655657339099 1106 2 0.20484217649207181 1106 3 0.16060099782846393 1106 4 0.3812028814139694 1106 5 0.17790634416898143 1106 6 0.2656490111266123 1106 7 0.2308372971816013 1106 8 0.3813869597951012 1106 9 0.22936678888922302 1106 10 0.3699308076831329 1106 11 0.30482705973232527 1106 12 0.12841647419303825 1106 13 0.2121542009296533 1106 14 0.5272730492974631 1106 15 0.40347116124986115 1106 16 0.5032734145663724 1106 17 0.42424423302945513 1106 18 0.3256383320288876 1106 19 0.38043035279340387 1106 20 0.31423193754025647 1106 21 0.41608713472885483 1106 22 0.20616887885465474 1106 23 0.32048167400500915 1106 24 0.4002150619616245 1106 25 0.3751491611184292 1106 26 0.2757622215367874 1106 27 0.33197993704306583 1106 28 0.476986118949668 1106 29 0.41954801659880303 1106 30 0.5208552050402417 1106 31 0.4475836831858402 1106 32 0.34921351783288657 1106 33 0.43626129002396397 1106 34 0.4582055702714527 1106 35 0.31951486734296 1106 36 0.30862364134681286 1106 37 0.22101967164621072 1106 38 0.25830005620725416 1106 39 0.38993202919187553 1106 40 0.38510763028436656 1106 41 0.4959358414357453 1106 42 0.37487512638004006 1106 43 0.38988182077877553 1106 44 0.35133796292130626 1106 45 0.380653233148152 1106 46 0.2877154765642089 1107 1 0.5935844032237867 1107 2 0.557342713620006 1107 3 0.6286005681261173 1107 4 0.6926544490011963 1107 5 0.6680231901537397 1107 6 0.5644739404736328 1107 7 0.530629056812721 1107 8 0.6477487030644724 1107 9 0.7209999883537463 1107 10 0.7014833553222459 1107 11 0.7244314030314456 1107 12 0.6208016527674189 1107 13 0.7426919899457971 1107 14 0.8463518335703597 1107 15 0.86865700751558 1107 16 0.9075861387139952 1107 17 0.827755397027012 1107 18 0.8509907705897177 1107 19 0.8369023312421788 1107 20 0.8673373633587502 1107 21 0.816334213579232 1107 22 0.8739635141861015 1107 23 0.7148410043304239 1107 24 0.8582942924638814 1107 25 0.7229105771529376 1107 26 0.6935320663481335 1107 27 0.8265962624269036 1107 28 0.8396755598408063 1107 29 0.8609184365502145 1107 30 0.8567543572044585 1107 31 0.8805501168216168 1107 32 0.9750006168462235 1107 33 0.834277222064884 1107 34 0.8338315309041064 1107 35 0.8739383224803845 1107 36 0.9296303017332193 1107 37 0.9613513688052109 1107 38 0.8435257325752801 1107 39 0.9353730525445485 1107 40 1.0315328372852122 1107 41 1.2171581793470594 1107 42 1.101749702875317 1107 43 0.9469867838408987 1107 44 0.9152644507656902 1107 45 1.0056966165583319 1107 46 0.9229147097107884 1108 1 0.23567419253872046 1108 2 0.2505864859556297 1108 3 0.17475319997262417 1108 4 0.20465894042904154 1108 5 0.2427906142806293 1108 6 0.19421720846008128 1108 7 0.1532079801008095 1108 8 0.22106996947589153 1108 9 0.07893004301610063 1108 10 0.08752831241019976 1108 11 0.040914524056518076 1108 12 0.014424110620147701 1108 13 0.08237606490876459 1108 14 0.13157868367564163 1108 15 0.16776661494374423 1108 16 0.06642313002812728 1108 17 0.06457409085628996 1108 18 0.1459190959274892 1108 19 0.07136182143479175 1108 20 0.19492441598854526 1108 21 0.17104998985332998 1108 22 0.2186968780211913 1108 23 0.0749126477774098 1108 24 0.20525107361915218 1108 25 0.11243335848479319 1108 26 0.07740132084781237 1108 27 0.1086222121061482 1108 28 0.15768778194708233 1108 29 0.19128386896608873 1108 30 -0.05290426395573178 1108 31 0.08748355789214202 1108 32 0.2949499678812799 1108 33 0.16738201521037865 1108 34 0.3160774142821694 1108 35 0.29851537820494656 1108 36 0.27833290695757995 1108 37 0.33895750732776975 1108 38 0.22888573799781398 1108 39 0.2167367930941353 1108 40 0.25297623914046596 1108 41 0.2398799792716062 1108 42 0.22288157658378793 1108 43 0.4035215616374046 1108 44 0.21847774810338905 1108 45 0.05622205832094096 1108 46 0.1595318422426928 1109 1 0.017873874680965685 1109 2 -0.0016704983062005851 1109 3 0.12005541469878267 1109 4 0.15520735743589142 1109 5 0.0736749488426085 1109 6 0.04322561054512164 1109 7 0.1323984008117094 1109 8 -0.029566848036097326 1109 9 0.006243813309644963 1109 10 0.05928019394872308 1109 11 0.027911619343797156 1109 12 0.020889393182073945 1109 13 0.1554605442372079 1109 14 0.12978066846446978 1109 15 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-0.9300325059338803 2215 29 -0.7182735542293059 2215 30 -0.8670929034961006 2215 31 -0.8464899507570749 2215 32 -0.8434263888593065 2215 33 -0.6798416419831028 2215 34 -0.6456818881911701 2215 35 -0.8412522079121345 2215 36 -0.9572559240970193 2215 37 -0.724778176112423 2215 38 -0.6529181534858992 2215 39 -0.828840665071013 2215 40 -0.7189503790024006 2215 41 -0.6061654180729263 2215 42 -0.6355578159701167 2215 43 -0.6370982192731631 2215 44 -0.6294026311090448 2215 45 -0.6769269257391954 2215 46 -0.7239168231944504 2216 1 -1.4204372555506133 2216 2 -1.2922810550866615 2216 3 -1.139050333989811 2216 4 -1.0449666822901449 2216 5 -1.1108349288895951 2216 6 -1.2848411307565613 2216 7 -1.5128793049927083 2216 8 -1.4532325353219093 2216 9 -1.7288255825867296 2216 10 -1.7402155797214078 2216 11 -1.5364683152446474 2216 12 -1.8686019317197875 2216 13 -1.7702539230594567 2216 14 -3.6233952143227057 2216 15 -2.556042586881908 2216 16 -2.1441415681332927 2216 17 -2.1306975191151736 2216 18 -2.313578751278854 2216 19 -2.1364717621463 2216 20 -2.000707153015199 2216 21 -2.043833222333575 2216 22 -2.053413456382742 2216 23 -2.129626017987704 2216 24 -2.0988003547818153 2216 25 -2.117776949071363 2216 26 -1.9552966814789983 2216 27 -2.1585984467580914 2216 28 -2.1733838419454443 2216 29 -2.416564630044519 2216 30 -2.2047777413389724 2216 31 -2.205685772007328 2216 32 -2.147288539780277 2216 33 -1.99593656842044 2216 34 -1.898574111566269 2216 35 -1.9406016283677872 2216 36 -2.192427481639079 2216 37 -2.206118710517053 2216 38 -2.2630847891134636 2216 39 -2.143502649267674 2216 40 -2.013246279180656 2216 41 -2.1334532214801314 2216 42 -2.017889650558317 2216 43 -2.065096374663767 2216 44 -1.9625784631786445 2216 45 -2.0640430385796336 2216 46 -2.248183014519462 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84038.0 3 2677.0 4 7608.0 5 5794.0 6 4673.0 7 3892.0 8 3334.0 9 2919.0 10 2826.0 11 2706.0 12 2594.0 13 2812.0 14 2933.0 15 2922.0 16 3161.0 17 3499.0 18 3890.0 19 4836.0 20 6373.0 21 8868.0 22 11256.0 23 12826.0 24 13907.0 25 14743.0 26 15976.0 27 18473.0 28 21142.0 29 22928.0 30 26021.0 31 31126.0 32 38413.0 33 47412.0 34 61265.0 35 81402.0 36 111429.0 37 162756.0 38 294227.0 39 816298.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66437613610333 18.431926142506068 13.748234145316129 24.155463576074474 2 15.229400050232286 20.535681989891387 39.23997430307797 24.99494365679835 3 18.141000425964688 25.77142408758975 29.714297942937034 26.37327754350852 4 12.468507244671132 17.13175320582875 38.610207193671336 31.789532355828776 5 13.71149685780638 37.936620245639716 34.669166769818844 13.682716126735059 6 31.09506073204325 37.688236237592 18.028722654032297 13.187980376332451 7 27.41284805405419 32.530786092349366 22.338546453457436 17.717819400139007 8 26.267251540895792 34.500739579063676 20.426129926602187 18.80587895343834 9 26.664409316914085 15.854996677622452 19.511546853161096 37.96904715230237 10 14.345644375762173 29.018740246085066 34.264583455323574 22.37103192282919 11 33.79817765682004 23.683281948924492 24.302084314559142 18.21645607969633 12 23.181590209551644 25.08484155696032 30.63817653663144 21.0953916968566 13 27.500986529732096 22.612228895264913 26.257780855467665 23.629003719535323 14 22.27036770190763 21.587612750801316 27.37262309981644 28.76939644747461 15 23.253102289107733 29.32178112205333 24.03224517083657 23.392871418002365 16 23.178636985063303 28.626674236425988 25.511684330852795 22.683004447657915 17 22.160719122909565 28.27172613531519 26.829732762781905 22.737821978993335 18 22.162353724717835 28.065861683742938 27.59242418533413 22.179360406205095 19 23.097568315234575 27.615918352175488 27.678649169249475 21.607864163340455 20 23.374429900336622 27.11212473477668 27.604102222554893 21.909343142331807 21 23.50380544009646 26.8110529105621 27.488684499559294 22.19645714978214 22 23.343813885756038 26.59001861040604 27.355667517840278 22.710499985997643 23 23.226813309911147 26.82527559255582 27.097736704091247 22.850174393441787 24 22.83760492274478 26.947139558388855 27.321080783830503 22.894174735035858 25 23.11692120627026 26.763370330427943 27.370314134152913 22.749394329148885 26 22.861476717430346 27.04684518902592 27.42022783826569 22.671450255278046 27 22.950305355213857 27.10839231536718 27.15086854726703 22.790433782151936 28 22.963328818599347 26.901025489577073 27.24324572033769 22.89239997148589 29 23.03691313612721 27.129309269400466 27.268519579313978 22.565258015158342 30 23.147209931107522 27.008498266231484 27.183962768530446 22.660329034130545 31 23.17601046476873 26.733380076510127 27.223063226002242 22.8675462327189 32 22.971068362577267 27.018340682505578 27.206126460075154 22.804464494842 33 23.109569747820426 26.83054995167823 27.12725276717256 22.932627533328784 34 23.229762366063905 26.865328888443063 27.2165173127186 22.68839143277443 35 23.21029131186832 26.98322580119547 27.1011538722008 22.705329014735405 36 23.170485111706135 26.92132361442678 27.079779618309995 22.82841165555709 37 23.272075841219035 26.76955290976058 27.091583761375343 22.866787487645045 38 23.094934491412182 26.879348906560637 27.171397842453477 22.854318759573705 39 23.252772735771053 27.01027482573449 27.02800321760124 22.708949220893217 40 23.232434342832494 26.717021949464442 27.335310267069385 22.71523344063368 41 23.162781408637848 26.869825005451386 27.10698572042853 22.860407865482237 42 23.140251233956526 26.90223925917774 27.067157396198883 22.890352110666846 43 23.13890985542765 26.705241702598393 27.092519987817337 23.063328454156622 44 23.202580424345957 26.892866347016753 27.004611393105222 22.899941835532072 45 23.30599179499194 26.893480048995766 26.838576270668497 22.961951885343797 46 23.273081559656998 26.77308639799784 26.82080771771388 23.13302432463128 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 527.0 1 543.0 2 559.0 3 675.0 4 791.0 5 867.5 6 944.0 7 944.0 8 917.0 9 890.0 10 1065.0 11 1240.0 12 1731.5 13 2223.0 14 4014.5 15 5806.0 16 8119.5 17 10433.0 18 11070.5 19 11708.0 20 11708.0 21 13383.0 22 15058.0 23 18719.5 24 22381.0 25 27940.5 26 33500.0 27 38684.0 28 43868.0 29 50237.5 30 56607.0 31 56607.0 32 62887.0 33 69167.0 34 77016.5 35 84866.0 36 92206.0 37 99546.0 38 105253.5 39 110961.0 40 117806.5 41 124652.0 42 129521.5 43 134391.0 44 134391.0 45 137669.0 46 140947.0 47 143970.0 48 146993.0 49 148191.0 50 149389.0 51 146149.0 52 142909.0 53 137627.0 54 132345.0 55 123785.0 56 115225.0 57 115225.0 58 105452.0 59 95679.0 60 84959.5 61 74240.0 62 63274.0 63 52308.0 64 43702.0 65 35096.0 66 28178.0 67 21260.0 68 16920.0 69 12580.0 70 12580.0 71 9745.5 72 6911.0 73 5391.5 74 3872.0 75 2927.0 76 1982.0 77 1522.0 78 1062.0 79 771.0 80 480.0 81 480.0 82 359.5 83 239.0 84 180.5 85 122.0 86 98.0 87 74.0 88 58.0 89 42.0 90 32.5 91 23.0 92 18.5 93 14.0 94 14.0 95 12.0 96 10.0 97 10.5 98 11.0 99 17.5 100 24.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19633851081109294 2 0.054685570697902953 3 0.06878976351291145 4 0.061203031637690274 5 0.042567166762985914 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0183532718417682E-4 16 0.0 17 3.564236451446189E-4 18 0.0010692709354338567 19 4.073413087367073E-4 20 0.0022403771980518904 21 0.0028513891611569512 22 0.0 23 2.545883179604421E-4 24 5.091766359208842E-4 25 8.65600281065503E-4 26 9.165179446575915E-4 27 0.025662502450412558 28 7.637649538813262E-4 29 0.0010183532718417683 30 0.0012729415898022102 31 0.0016293652349468292 32 7.637649538813262E-4 33 0.0018839535529072715 34 0.0026477185067885975 35 0.002342212525236067 36 0.032281798717384055 37 0.008554167483470853 38 0.012372992252877486 39 0.05101949891927259 40 0.01568264038636323 41 0.05860623079449376 42 0.05921724275759883 43 0.025917090768373 44 0.028361138620793248 45 0.02647718506788597 46 0.02403313721546573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 46 1963955.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.90199716400014 #Duplication Level Percentage of deduplicated Percentage of total 1 81.13147417034298 55.90120603196122 2 11.499461198077409 15.846716857149184 3 3.472292858379951 7.1774373804202 4 1.4305660696013789 3.942754370823561 5 0.7505450781901885 2.585702742445731 6 0.4358379800526539 1.8018064359330905 7 0.27287786877744863 1.3161281098435518 8 0.18924433497849244 1.043145010559294 9 0.1392244911087242 0.8633560942379407 >10 0.652059821437161 7.541191580247303 >50 0.020551601519151225 0.9352341462078541 >100 0.005642058364939228 0.578515185917902 >500 0.0 0.0 >1k 2.22469169453253E-4 0.4668060542532098 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4701 0.23936393654640764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2259 0.11502300205452771 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2176 0.11079683597638439 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 5.091766359208841E-5 16 0.0 0.0 0.0 0.0 5.091766359208841E-5 17 0.0 0.0 0.0 0.0 5.091766359208841E-5 18 0.0 0.0 0.0 5.091766359208841E-5 5.091766359208841E-5 19 0.0 0.0 0.0 5.091766359208841E-5 5.091766359208841E-5 20 0.0 0.0 0.0 5.091766359208841E-5 5.091766359208841E-5 21 0.0 0.0 0.0 5.091766359208841E-5 5.091766359208841E-5 22 0.0 0.0 0.0 5.091766359208841E-5 5.091766359208841E-5 23 0.0 0.0 0.0 5.091766359208841E-5 5.091766359208841E-5 24 0.0 0.0 0.0 1.5275299077626523E-4 5.091766359208841E-5 25 0.0 0.0 0.0 2.0367065436835364E-4 5.091766359208841E-5 26 0.0 0.0 0.0 3.564236451446189E-4 5.091766359208841E-5 27 0.0 0.0 0.0 3.564236451446189E-4 5.091766359208841E-5 28 0.0 0.0 0.0 6.619296266971494E-4 5.091766359208841E-5 29 0.0 0.0 0.0 0.0012729415898022104 5.091766359208841E-5 30 0.0 0.0 0.0 0.0021385418708677133 5.091766359208841E-5 31 0.0 0.0 0.0 0.0036151541150382773 5.091766359208841E-5 32 0.0 0.0 0.0 0.0060592019674585215 5.091766359208841E-5 33 0.0 0.0 0.0 0.008605085147062942 5.091766359208841E-5 34 0.0 0.0 0.0 0.012118403934917043 5.091766359208841E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2930 0.0 24.762037 1 GGTATCA 1155 0.0 22.558323 1 ATCAACG 3840 0.0 18.443924 3 TCAACGC 3870 0.0 18.349842 4 TATCAAC 3895 0.0 18.296938 2 CAACGCA 3970 0.0 17.782776 5 AACGCAG 4075 0.0 17.416515 6 GTATTAG 340 0.0 15.32639 1 TAGTCGG 95 6.887634E-4 14.7423525 40 ACGCAGA 4740 0.0 14.593461 7 GTGGTAT 565 0.0 14.543898 1 CGCAGAG 4765 0.0 14.516894 8 GACCGTC 100 0.001021405 13.994538 7 CGAATCG 130 3.3537926E-5 13.852388 37 CGTGCCG 145 6.169781E-6 13.787722 10 TGGACCG 190 3.7773134E-8 13.683749 5 TACCGAT 135 4.820103E-5 13.328131 7 AGAGTAC 4915 0.0 13.260339 11 GAGTACT 2400 0.0 12.994928 12 CCCTATA 170 2.1978285E-6 12.953276 2 >>END_MODULE