FastQCFastQC Report
Mon 6 Feb 2017
SRR2077337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963955
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.040.757761
GTATCAA40850.037.3360631
TATCAAC55100.027.4166162
TCAACGC55900.027.2081094
ATCAACG55800.027.0706043
CAACGCA57400.026.4957375
AACGCAG59900.025.21646
ACGCAGA68300.021.8234637
CGCAGAG69600.021.2595358
AGAGTAC68900.020.27461611
CGTATAC1055.856781E-419.7973733
GAGTACT47450.019.40849512
GTACATG49300.019.335421
TATGCCG3400.019.26898443
CAGAGTA75200.018.89714610
GCAGAGT77200.018.8538889
ACCGTCG1301.1839336E-418.5720758
AGTACTT50250.018.39566613
ACGCGTA1150.001114834317.98980565
CCCTATA2902.0008883E-1117.9214122
Produced by FastQC (version 0.11.5)