FastQCFastQC Report
Mon 6 Feb 2017
SRR2077336_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077336_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741933
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28560.16395578934436628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.022.7741
GTATCAA22900.021.9705921
GTATCCG500.004494853319.9919479
TCAACGC31150.016.374264
ATCAACG31200.016.3508383
TATCAAC30750.016.3387992
CAACGCA32000.015.8740775
AACGCAG32800.015.4206196
GGATCGT956.927743E-414.7309086
GTACATG47800.013.1256241
TAATACC2608.367351E-1113.0784174
TATACAG7200.012.7770355
GTAATAC2201.8579158E-812.7309193
ACGCAGA39550.012.6876857
CCTATAC3005.456968E-1212.6702963
GTATATG4750.012.6599261
TATACTG5250.012.5706985
TACATGG48600.012.56189252
GTATTAC2252.4741894E-812.4723721
CGCAGAG40250.012.4173598
Produced by FastQC (version 0.11.5)