FastQCFastQC Report
Mon 6 Feb 2017
SRR2077336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741933
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17950.038.63671
GTATCAA36750.036.3276981
TATCAAC49700.026.3637352
ATCAACG49950.026.2996623
TCAACGC50650.025.9361924
CAACGCA50800.025.859615
AACGCAG53700.024.6538896
ACGCAGA61550.021.4704697
CGCAGAG62900.020.9457768
GAATACG1507.338331E-720.69949223
AGAGTAC62150.020.42280411
GAGTACT38900.020.30460412
CAGAGTA66400.019.27090310
TAGATCG900.005582929619.256355
GCAGAGT66800.019.2065879
AGTACTT42000.018.80593113
ACGTAAT1702.4456876E-618.25534256
GTACTTT42700.018.08856414
GTACATG49750.017.2859441
GTATTAG3750.016.6446911
Produced by FastQC (version 0.11.5)