FastQCFastQC Report
Mon 6 Feb 2017
SRR2077335_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077335_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324634
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53990.23225161466278132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25660.11038296781342782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23860.10263981340718582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA300.00916706426.6564412
GTATCAA33500.024.3594021
GGTATCA14300.024.2564431
CGTTACG1005.2659743E-921.99771524
CGACTTA550.007792817418.17562915
TATCAAC44850.017.5898022
TCAACGC46300.017.067844
ATCAACG46250.017.0496433
CAACGCA47150.016.8000495
AACGCAG49350.015.9611136
TCGCGTC1557.8854646E-714.18871717
GCACCGT2751.8189894E-1213.8125866
TCGTTAC1601.1249958E-613.74857323
GTTACGG1752.0603511E-713.71315725
ACGCAGA58000.013.3738947
GTCTAGG3900.012.8526421
CGACACG1253.7498242E-412.79894627
GTAGCCC4850.012.7923623
TAAGGCG1100.002086482712.72923855
CGCAGAG60650.012.6906558
Produced by FastQC (version 0.11.5)