FastQCFastQC Report
Mon 6 Feb 2017
SRR2077335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324634
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44400.038.7406041
GGTATCA20650.035.938621
TATCAAC61250.027.6752762
ATCAACG60700.027.6381073
TCAACGC60900.027.603664
CAACGCA62200.027.137015
AACGCAG65950.025.5392046
CGTATAC1253.3662727E-622.1835783
ACGCAGA77350.021.639617
CGCAGAG79450.021.0513218
GTACATG62250.019.8325391
TACATGG61850.019.7284132
GAGTACT52900.019.68841712
AGAGTAC80800.019.63387511
ATGCGCG900.00575333619.15674251
CAGAGTA87100.018.60889410
AGTACTT56200.018.593713
GCAGAGT90700.018.1360459
ACATGGG67050.018.0934283
TAATTCG1552.2589269E-517.7960859
Produced by FastQC (version 0.11.5)