FastQCFastQC Report
Mon 6 Feb 2017
SRR2077334_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077334_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2479401
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62690.2528433278844366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28130.11345482235427026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27770.11200285875499769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.024.277951
GTATCAA36400.022.853361
TATCAAC50100.016.1401082
ATCAACG51050.015.7151523
TCAACGC51000.015.64831354
CAACGCA51450.015.3550155
AACGCAG53650.014.7575786
CGCACGA1252.3220946E-514.403769535
GTATAGA4600.013.5085021
TCTATAC4800.012.9208323
CGTGCCG1406.776376E-512.85298710
ACGCAGA62350.012.5380487
GTACATG62050.012.5341021
ACCGTGC3850.012.4635038
GCACCGT2252.5664121E-812.4404226
GTATTAG3550.012.4221751
TGCGACT1150.002923069812.16997812
TACATGG63350.012.1008252
CGCAGAG64350.012.0240868
GTTTAAG5100.011.7911011
Produced by FastQC (version 0.11.5)