##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077334_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2479401
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.070613426388068	31.0	31.0	34.0	26.0	34.0
2	30.11525929044959	31.0	31.0	34.0	26.0	34.0
3	30.182614671850178	33.0	31.0	34.0	26.0	34.0
4	33.566813516651806	37.0	35.0	37.0	28.0	37.0
5	33.58578826095496	37.0	35.0	37.0	28.0	37.0
6	33.64909427720647	37.0	35.0	37.0	28.0	37.0
7	33.52625694673834	37.0	35.0	37.0	28.0	37.0
8	33.48487880742163	37.0	35.0	37.0	27.0	37.0
9	34.96538276785401	39.0	35.0	39.0	27.0	39.0
10	34.87870941408832	39.0	35.0	39.0	27.0	39.0
11	34.931014789459226	39.0	35.0	39.0	27.0	39.0
12	34.8606639264887	39.0	35.0	39.0	27.0	39.0
13	34.831638770816014	39.0	35.0	39.0	27.0	39.0
14	35.87855615126396	40.0	36.0	41.0	26.0	41.0
15	36.0120464579953	40.0	37.0	41.0	27.0	41.0
16	36.00098531863139	40.0	37.0	41.0	27.0	41.0
17	36.026498335686725	40.0	37.0	41.0	27.0	41.0
18	35.992648224309015	40.0	37.0	41.0	27.0	41.0
19	36.08724889600351	40.0	37.0	41.0	27.0	41.0
20	36.077248093390295	40.0	37.0	41.0	27.0	41.0
21	36.082330772634194	40.0	37.0	41.0	27.0	41.0
22	36.03117648173894	40.0	37.0	41.0	27.0	41.0
23	35.98752238948036	40.0	37.0	41.0	27.0	41.0
24	36.01120351246128	40.0	37.0	41.0	27.0	41.0
25	35.95503349397697	40.0	37.0	41.0	27.0	41.0
26	35.97958539179423	40.0	37.0	41.0	27.0	41.0
27	35.95145924358343	40.0	37.0	41.0	27.0	41.0
28	35.8795277569058	40.0	36.0	41.0	27.0	41.0
29	35.84139233629413	40.0	36.0	41.0	26.0	41.0
30	35.75048812192945	40.0	36.0	41.0	26.0	41.0
31	35.7174797461161	40.0	36.0	41.0	25.0	41.0
32	35.684243089359086	40.0	36.0	41.0	25.0	41.0
33	35.631608198915785	40.0	36.0	41.0	25.0	41.0
34	35.56903139104969	40.0	36.0	41.0	25.0	41.0
35	35.54086975039536	40.0	36.0	41.0	25.0	41.0
36	35.4791947732537	40.0	36.0	41.0	25.0	41.0
37	35.39149818847375	40.0	36.0	41.0	24.0	41.0
38	35.357202404935705	40.0	35.0	41.0	24.0	41.0
39	35.27149259034743	40.0	35.0	41.0	24.0	41.0
40	35.24567587090591	40.0	35.0	41.0	24.0	41.0
41	35.105050776377034	40.0	35.0	41.0	23.0	41.0
42	35.04765021874235	40.0	35.0	41.0	23.0	41.0
43	34.9505190971529	40.0	35.0	41.0	22.0	41.0
44	34.86508515564848	40.0	35.0	41.0	21.0	41.0
45	34.75512512901302	40.0	35.0	41.0	19.0	41.0
46	34.64711799341857	40.0	35.0	41.0	17.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3979287020309066
1101	2	0.3865338602861996
1101	3	0.4567292404148269
1101	4	0.4422309266818232
1101	5	0.2520255507783915
1101	6	0.3274020885103823
1101	7	0.41024765979186384
1101	8	0.510421764520899
1101	9	0.5374744520423107
1101	10	0.4779703751728306
1101	11	0.5554930039783059
1101	12	0.5352551840941331
1101	13	0.6105437612270777
1101	14	0.7682500842633218
1101	15	0.6093301175833474
1101	16	0.6177120310858015
1101	17	0.5624407781083036
1101	18	0.5784064891497849
1101	19	0.5492747284955541
1101	20	0.5457872434217279
1101	21	0.5531620479899573
1101	22	0.5767265650775002
1101	23	0.6323778871865429
1101	24	0.5870985606501904
1101	25	0.5866617433253012
1101	26	0.636752343816724
1101	27	0.6876369146812067
1101	28	0.5809876205331506
1101	29	0.6128496327366832
1101	30	0.6862609456632427
1101	31	0.7867727819632364
1101	32	0.6337156105394754
1101	33	0.6480709013968067
1101	34	0.6839009000440086
1101	35	0.6131532135902873
1101	36	0.6241086831992604
1101	37	0.6236914181207425
1101	38	0.5966079043873407
1101	39	0.6512289720836506
1101	40	0.5825911688264043
1101	41	0.5958162778051417
1101	42	0.5611796898981254
1101	43	0.5317439846764316
1101	44	0.47783884144426025
1101	45	0.45585076158521076
1101	46	0.5928684649301132
1102	1	0.6206981019363269
1102	2	0.7189313688852508
1102	3	0.6987297981911595
1102	4	0.8017012008410163
1102	5	0.6429398593580373
1102	6	0.7335812802197239
1102	7	0.7241439699801759
1102	8	0.7196909562706608
1102	9	0.8839166069990441
1102	10	0.8755921462339415
1102	11	0.8234665378955555
1102	12	0.8613837717478745
1102	13	0.8852910440883903
1102	14	1.1887293677228286
1102	15	1.0606144177963515
1102	16	1.073818671453445
1102	17	1.0150770796470425
1102	18	1.0020582265422178
1102	19	0.9905032914939653
1102	20	0.9509996228228061
1102	21	0.889731561875756
1102	22	0.9921559518064598
1102	23	1.0176780557319987
1102	24	0.9951422612132248
1102	25	1.1079401020078166
1102	26	1.024438825016027
1102	27	1.0627087586978448
1102	28	0.9860585349225701
1102	29	1.004467628702912
1102	30	0.9629446824413179
1102	31	1.0306540160231563
1102	32	1.046465245761837
1102	33	1.0864224702437895
1102	34	1.0622470528019647
1102	35	1.1157303019169973
1102	36	1.0503338712466785
1102	37	1.0995756754900583
1102	38	1.0057655408707689
1102	39	1.025288330802546
1102	40	1.0254106069273732
1102	41	1.124709900158237
1102	42	1.1141449986354104
1102	43	0.9946304367743579
1102	44	1.0519519568675832
1102	45	1.0612510873589258
1102	46	0.9632679217044711
1103	1	0.635337786702241
1103	2	0.5534149897904754
1103	3	0.6077374072362787
1103	4	0.6779179258297177
1103	5	0.7476965609558022
1103	6	0.7097535841652771
1103	7	0.6285630095226722
1103	8	0.6996778784670497
1103	9	0.8432284069987332
1103	10	0.8182223690070245
1103	11	0.7551789691089468
1103	12	0.7309102372151699
1103	13	0.7888305159128635
1103	14	1.0782932959719673
1103	15	0.9991670683813609
1103	16	0.9926781223410117
1103	17	0.8753715787376493
1103	18	0.8842764519060182
1103	19	0.844629788956837
1103	20	0.8449906671677425
1103	21	0.9091030196470342
1103	22	0.9174936526913129
1103	23	0.8312253090049424
1103	24	0.9025323851417184
1103	25	0.9012643647505243
1103	26	0.9071012608113094
1103	27	0.8494735399552695
1103	28	0.8256954627880759
1103	29	0.9343999565053593
1103	30	0.9501928920092411
1103	31	0.9925983841027559
1103	32	0.9505432839049135
1103	33	0.971926221958114
1103	34	0.9662584593803203
1103	35	0.9934545466427807
1103	36	1.0311786165945023
1103	37	1.1062055161800473
1103	38	1.068782149060084
1103	39	1.1542162926066624
1103	40	1.0335688576701898
1103	41	1.1203596666371212
1103	42	1.0627931078676838
1103	43	1.081044586895736
1103	44	1.1113595106193799
1103	45	1.0915149421424957
1103	46	1.0680544444221027
1104	1	0.6111693909251841
1104	2	0.5282525262505047
1104	3	0.6461020345086652
1104	4	0.6474308037947338
1104	5	0.6801171758969531
1104	6	0.7081326664666818
1104	7	0.6374222913477823
1104	8	0.68763829299224
1104	9	0.8003896402072428
1104	10	0.7869904928001006
1104	11	0.7711001380634457
1104	12	0.7220001215172758
1104	13	0.7765297050291338
1104	14	1.0677857827255721
1104	15	0.9703383445422133
1104	16	1.0878901936711003
1104	17	0.9717672447175332
1104	18	0.816493351998794
1104	19	0.845717557962864
1104	20	0.9538044412960005
1104	21	0.8805157187678674
1104	22	0.9898636370951763
1104	23	0.9688436039791881
1104	24	0.9660198710561829
1104	25	0.8578332695339768
1104	26	0.8553971424785516
1104	27	0.966650548356057
1104	28	0.988236695128613
1104	29	1.061194961250358
1104	30	1.0788027418839121
1104	31	1.0833447376449072
1104	32	1.1421749965936883
1104	33	1.1633944316680171
1104	34	1.1161661845205089
1104	35	1.0861480651267357
1104	36	1.16155141552575
1104	37	1.1030423594784864
1104	38	1.1043629010190088
1104	39	1.049753063402342
1104	40	1.059790638421724
1104	41	1.1465526921083793
1104	42	1.1150979004852104
1104	43	1.0660620995442684
1104	44	0.9483720023987061
1104	45	0.9676626116598044
1104	46	0.9377527370413006
1105	1	0.31657135175209916
1105	2	0.1733326810777065
1105	3	0.42825412842626065
1105	4	0.2785238884583876
1105	5	0.3999251447361303
1105	6	0.5032083037773845
1105	7	0.37062458423838507
1105	8	0.4391242412684022
1105	9	0.5903087908961595
1105	10	0.4607135002644185
1105	11	0.48927108778025996
1105	12	0.5295773676781721
1105	13	0.5586002888645822
1105	14	0.8430094447428189
1105	15	0.5180371006594058
1105	16	0.5311700395091705
1105	17	0.4655655860772612
1105	18	0.25690169054012557
1105	19	0.4192502903291526
1105	20	0.4696698393821066
1105	21	0.4331931826236115
1105	22	0.4272951345387739
1105	23	0.5450534331061903
1105	24	0.47902986907686795
1105	25	0.4144226179942194
1105	26	0.475077092541639
1105	27	0.4707673319262682
1105	28	0.5220011978174739
1105	29	0.5344697681471331
1105	30	0.6968620048080183
1105	31	0.638883744789176
1105	32	0.5997397839984586
1105	33	0.6612388346149061
1105	34	0.6060676247467214
1105	35	0.7459701573856563
1105	36	0.7464091796832193
1105	37	0.7399446107599843
1105	38	0.6817324508001619
1105	39	0.6763996532167909
1105	40	0.5274026672121579
1105	41	0.5194302272554836
1105	42	0.6352708217271399
1105	43	0.6855259130384113
1105	44	0.6982471526267275
1105	45	0.7147538329790066
1105	46	0.7346374037212513
1106	1	0.13637580408903105
1106	2	0.23841805254526705
1106	3	0.24544685892773188
1106	4	0.3376914328499083
1106	5	0.36271507327169417
1106	6	0.2971160242303341
1106	7	0.2994003033147834
1106	8	0.36452741120834986
1106	9	0.36947862953879707
1106	10	0.4250787152461726
1106	11	0.4040234203930382
1106	12	0.41253808384206536
1106	13	0.3695995842017368
1106	14	0.7071479162054644
1106	15	0.5378564507308425
1106	16	0.6264572901769583
1106	17	0.5201051492458859
1106	18	0.4967018821249951
1106	19	0.5021098508757831
1106	20	0.4362975733026033
1106	21	0.4260978386125771
1106	22	0.43917071461930846
1106	23	0.4907717497217803
1106	24	0.5257050671794943
1106	25	0.48326713480854977
1106	26	0.5264711480476194
1106	27	0.459603714577554
1106	28	0.4861089799470477
1106	29	0.4847803114228171
1106	30	0.6431102338398347
1106	31	0.6276800525346573
1106	32	0.5669164994353437
1106	33	0.635765347368114
1106	34	0.5373665731156407
1106	35	0.5172474742440798
1106	36	0.37289273332921624
1106	37	0.3670313736759496
1106	38	0.3480617830986006
1106	39	0.3762664674703444
1106	40	0.3834667155523306
1106	41	0.4176609723060807
1106	42	0.3766372121401531
1106	43	0.37414478876597457
1106	44	0.42505711563565285
1106	45	0.43560246752715415
1106	46	0.30832755765954545
1107	1	0.5467450928463897
1107	2	0.4293530731948998
1107	3	0.41536473842542065
1107	4	0.5650529586195816
1107	5	0.5129337377596812
1107	6	0.5020276096094349
1107	7	0.47409606344005795
1107	8	0.4811205619905152
1107	9	0.5245092164925182
1107	10	0.5132324452900505
1107	11	0.4890644227006433
1107	12	0.5965728963294836
1107	13	0.5424344870810955
1107	14	0.8458530515978211
1107	15	0.7851966243007595
1107	16	0.6964464402464188
1107	17	0.6504995855058056
1107	18	0.7688398689051752
1107	19	0.7119544607490766
1107	20	0.751489648942119
1107	21	0.615273837812957
1107	22	0.6158621956130972
1107	23	0.6477861791153856
1107	24	0.8258974397797232
1107	25	0.6121138987334689
1107	26	0.5680259474397928
1107	27	0.7624355609344278
1107	28	0.6959480851826783
1107	29	0.7079264436181418
1107	30	0.6781668084776484
1107	31	0.6534100326745005
1107	32	0.7282177378339583
1107	33	0.73994872712872
1107	34	0.7407961072349778
1107	35	0.7732855547479573
1107	36	0.7993119520473044
1107	37	0.8142507349351789
1107	38	0.6541890242466408
1107	39	0.8504463851035808
1107	40	0.8411215660796856
1107	41	0.7949200104141099
1107	42	0.7579166637623516
1107	43	0.7320448853868555
1107	44	0.7926301643502498
1107	45	0.7766337469063487
1107	46	0.8499585528256048
1108	1	0.30249240472391037
1108	2	0.3330754198743513
1108	3	0.26207799204466653
1108	4	0.24334792532343386
1108	5	0.18791291806287092
1108	6	0.19603799026417335
1108	7	0.1500478539339838
1108	8	0.32793983473008126
1108	9	0.20543161356945916
1108	10	0.30255555161954817
1108	11	0.2889925140799079
1108	12	0.07391399825227296
1108	13	0.12915505448313525
1108	14	0.2777058048682761
1108	15	0.28404424255816707
1108	16	0.2568711927916425
1108	17	0.23977536735840488
1108	18	0.4130996249690071
1108	19	0.281529124330433
1108	20	0.3660947814967557
1108	21	0.3475847780642667
1108	22	0.40676483318443246
1108	23	0.22766774500242803
1108	24	0.33671292656114105
1108	25	0.2694232256503426
1108	26	0.22606665263595715
1108	27	0.21941791873820193
1108	28	0.25857465511074196
1108	29	0.33869718725775755
1108	30	0.18390413304673103
1108	31	0.23344063993358333
1108	32	0.37246059229889994
1108	33	0.27833072170177076
1108	34	0.2992787124167009
1108	35	0.2208998172390082
1108	36	0.27637955536985004
1108	37	0.3334909005093891
1108	38	0.12277817096173749
1108	39	0.21921929829389342
1108	40	0.22095119796286156
1108	41	0.2911724649872198
1108	42	0.2014069361239379
1108	43	0.30214503504283385
1108	44	0.2505789863088097
1108	45	0.1997806402388207
1108	46	0.21505306349358477
1109	1	0.3154089787964338
1109	2	0.1929579779944035
1109	3	0.1818773623953298
1109	4	0.21371166442192902
1109	5	0.20393626030263334
1109	6	0.2237986531952103
1109	7	0.16035183100927242
1109	8	0.263017307529374
1109	9	0.31345210230722387
1109	10	0.4029729738040686
1109	11	0.28618035323886204
1109	12	0.3109473615362006
1109	13	0.31608690528464933
1109	14	0.5548324096541322
1109	15	0.3471845671179281
1109	16	0.22872857043208938
1109	17	0.38199077050072106
1109	18	0.31177201035988844
1109	19	0.3607882466505643
1109	20	0.18617087671493948
1109	21	0.30079413001330124
1109	22	0.20831115767990838
1109	23	0.19299047119012158
1109	24	0.23547829524332542
1109	25	0.27595719037272914
1109	26	0.3442947015058877
1109	27	0.21865372518901438
1109	28	0.22792326938500906
1109	29	0.27387272515305483
1109	30	0.31375536802141824
1109	31	0.36924294289759274
1109	32	0.3975807769799218
1109	33	0.25459278789763573
1109	34	0.3936107421526813
1109	35	0.2857580664776336
1109	36	0.23745073387331672
1109	37	0.24509826434655224
1109	38	0.2703370230672064
1109	39	0.3476377390368981
1109	40	0.4091514397468927
1109	41	0.26331305022982576
1109	42	0.2911529804883699
1109	43	0.3045112331854156
1109	44	0.1722398079844325
1109	45	0.06033743023035498
1109	46	0.0248099362223968
1110	1	0.19293830694125447
1110	2	0.16434700004117175
1110	3	0.1799010959112337
1110	4	0.15208117621642714
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2216	12	-3.0417849165493607
2216	13	-2.9770301438127333
2216	14	-4.867086740509556
2216	15	-3.7980438402433876
2216	16	-3.3901550959256994
2216	17	-3.2673654778118006
2216	18	-3.5250803951758485
2216	19	-3.183629878570123
2216	20	-3.1242919503585256
2216	21	-3.207627049501056
2216	22	-3.288734308961388
2216	23	-3.2283402025163923
2216	24	-3.175938804183801
2216	25	-3.1879979886939864
2216	26	-3.2916821220238432
2216	27	-3.2908355336271313
2216	28	-3.3146356201819103
2216	29	-3.5340262255532053
2216	30	-3.5287159366125422
2216	31	-3.323376106630903
2216	32	-3.5472689057434152
2216	33	-3.3296583767219445
2216	34	-3.207305254321568
2216	35	-3.2314335143842357
2216	36	-3.4593604357816616
2216	37	-3.423254242164603
2216	38	-3.573574183697147
2216	39	-3.4949699006342207
2216	40	-3.3963100057495943
2216	41	-3.6432382735931057
2216	42	-3.5223179661749846
2216	43	-3.492435105003729
2216	44	-3.4301270265857546
2216	45	-3.5674534585550077
2216	46	-3.6536082759999786
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	99402.0
3	3134.0
4	9285.0
5	6920.0
6	5341.0
7	4337.0
8	3792.0
9	3375.0
10	3214.0
11	3131.0
12	3123.0
13	3255.0
14	3329.0
15	3636.0
16	3733.0
17	4211.0
18	4641.0
19	5870.0
20	7800.0
21	10800.0
22	13659.0
23	15660.0
24	16646.0
25	17351.0
26	19111.0
27	21825.0
28	24268.0
29	26648.0
30	30198.0
31	36746.0
32	44952.0
33	55755.0
34	72411.0
35	96714.0
36	132592.0
37	192854.0
38	345334.0
39	1124348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.01466077757079	18.1138594456545	13.69182463641029	24.179655140364417
2	15.238804313832311	20.260968695459177	39.197159085177006	25.303067905531513
3	18.07417482075797	25.511553619246147	29.682520143918286	26.731751416077604
4	12.55662403898034	16.998652524428294	38.31862974769236	32.126093688899005
5	13.892345408826955	37.76597719181022	34.51792440954032	13.823752989822498
6	31.51757218779859	37.32308731020113	17.89448338530153	13.26485711669875
7	27.71879175655733	32.42638040397661	22.13490274465486	17.719925094811206
8	26.422873911884366	34.23056617303937	20.411099293740705	18.935460621335558
9	26.716614214481645	15.836284651010466	19.343906048275368	38.103195086232525
10	14.434050805012985	28.952033172528363	34.06488099343349	22.549035029025156
11	34.074399421473174	23.49857889062721	24.071217201251432	18.355804486648186
12	23.33563630893107	24.962238863338364	30.474941326554276	21.22718350117629
13	27.62667273264793	22.43263594715014	26.10094131606787	23.839750004134064
14	22.33797598694201	21.367902973339127	27.347532730687774	28.94658830903109
15	23.391797770346763	28.99234854898304	24.07817378324344	23.537679897426756
16	23.282397643624407	28.4365054301422	25.4492516539277	22.83184527230569
17	22.20050544608884	28.067826251091997	26.782391181070857	22.9492771217483
18	22.21108596153567	27.783549811485734	27.662955535801576	22.342408691177017
19	23.27052497505439	27.368865133980318	27.54943345027051	21.811176440694783
20	23.44953564517209	26.824931010255092	27.60291592084067	22.122617423732148
21	23.66283609588312	26.654182221806344	27.408492218934626	22.274489463375915
22	23.35108358833444	26.495472091847986	27.365278952456663	22.788165367360907
23	23.193551977981734	26.516901697026213	27.300358635732252	22.989187689259804
24	22.865767091286894	26.603739949148824	27.432854506266054	23.097638453298227
25	23.181406056655916	26.48125263169782	27.37639288405042	22.96094842759584
26	22.909111881999962	26.816284173237843	27.422522242375035	22.85208170238716
27	23.08837726437819	26.87606075752863	27.108514663113294	22.927047314979884
28	23.056586164241402	26.776295915079007	27.143564114839673	23.02355380583991
29	23.208546961664673	26.871351916443764	27.112419859126298	22.807681262765268
30	23.245457834401275	26.84486720263421	27.16172073732979	22.747954225634725
31	23.25939137130013	26.477498177969704	27.170898173210446	23.092212277519717
32	23.04050593089429	26.752830333267458	27.199512783386236	23.007150952452015
33	23.240947110036647	26.572715532227086	27.0397689094102	23.146568448326068
34	23.372646103994317	26.639998354405414	27.15142333848388	22.835932203116386
35	23.381418284219894	26.59240562693144	27.085677686437077	22.94049840241159
36	23.29298846540226	26.76026676026676	27.03767807216083	22.90906670217015
37	23.34523359592968	26.440807722760855	27.092747427917768	23.121211253391696
38	23.20784984738604	26.60287011860204	27.179322109239084	23.009957924772834
39	23.33646601750564	26.772263276211948	26.9979940867728	22.893276619509614
40	23.458461424273093	26.528932854060432	27.18894827136226	22.82365745030421
41	23.30543819923639	26.66592950442116	27.029089525260787	22.999542771081664
42	23.258545947913564	26.71146968592688	27.00917448964026	23.020809876519298
43	23.20759687414399	26.513337655301395	27.091397521955102	23.187667948599508
44	23.292741719129157	26.73285580866201	26.880228179065703	23.09417429314313
45	23.43955874604741	26.712130368725578	26.706764769718983	23.14154611550803
46	23.47091948352044	26.517240391647935	26.699824395013998	23.31201572981763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	725.0
1	701.5
2	678.0
3	873.5
4	1069.0
5	1195.5
6	1322.0
7	1322.0
8	1243.0
9	1164.0
10	1450.5
11	1737.0
12	2434.5
13	3132.0
14	5533.5
15	7935.0
16	10992.0
17	14049.0
18	14651.0
19	15253.0
20	15253.0
21	17032.5
22	18812.0
23	23510.0
24	28208.0
25	34738.5
26	41269.0
27	47324.0
28	53379.0
29	60382.0
30	67385.0
31	67385.0
32	75420.0
33	83455.0
34	93395.5
35	103336.0
36	111701.0
37	120066.0
38	127427.0
39	134788.0
40	144212.0
41	153636.0
42	159944.0
43	166252.0
44	166252.0
45	172169.5
46	178087.0
47	182352.5
48	186618.0
49	187989.5
50	189361.0
51	185778.5
52	182196.0
53	175768.5
54	169341.0
55	159615.0
56	149889.0
57	149889.0
58	136791.5
59	123694.0
60	110172.5
61	96651.0
62	83256.0
63	69861.0
64	58874.0
65	47887.0
66	38509.5
67	29132.0
68	23173.5
69	17215.0
70	17215.0
71	13540.0
72	9865.0
73	7736.0
74	5607.0
75	4331.0
76	3055.0
77	2338.0
78	1621.0
79	1227.5
80	834.0
81	834.0
82	613.5
83	393.0
84	293.5
85	194.0
86	150.5
87	107.0
88	75.0
89	43.0
90	35.5
91	28.0
92	23.5
93	19.0
94	19.0
95	16.5
96	14.0
97	14.0
98	14.0
99	19.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19242550922581705
2	0.05348065923987286
3	0.06477370945643726
4	0.05783655003769055
5	0.04004999594660162
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	8.066464440403146E-5
16	0.0
17	2.823262554141101E-4
18	5.243201886262044E-4
19	2.823262554141101E-4
20	0.0018956191434947392
21	0.0019762837878987707
22	0.0
23	2.0166161101007864E-4
24	3.6299089981814154E-4
25	5.243201886262044E-4
26	4.8398786642418876E-4
27	0.02540936298726991
28	5.646525108282202E-4
29	6.856494774342674E-4
30	8.87311088444346E-4
31	9.679757328483775E-4
32	4.43655544222173E-4
33	0.0010889726994544246
34	0.0021779453989088492
35	0.0015729605658786135
36	0.031096220417754127
37	0.007421147285170894
38	0.010889726994544246
39	0.04952809166407532
40	0.014842294570341788
41	0.05767522074888249
42	0.0581995409375087
43	0.023876734743593312
44	0.026054680142502163
45	0.026095012464704175
46	0.021860118633492527
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2479401.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.40704930239305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.0834616438276	53.18106385687963
2	11.96240318484525	15.887757961422444
3	3.6553575966132956	7.2822453640852824
4	1.5416543095134014	4.095068549564126
5	0.7935599740929018	2.634898816199654
6	0.48777514787674775	1.943502497611995
7	0.305279667489368	1.4190905350989234
8	0.2238897367358854	1.1894285428575795
9	0.14939480989981474	0.8928781655884772
>10	0.756646391950643	8.554260359900653
>50	0.030794383052936546	1.3812627287119492
>100	0.009539436304489309	1.0380344915810809
>500	6.0929449426275626E-5	0.020675800704813764
>1k	1.218588988356929E-4	0.22617950278653537
>5k	6.092944941784645E-5	0.25365282700693925
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6269	0.2528433278844366	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2813	0.11345482235427026	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2777	0.11200285875499769	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	4.033232220201573E-5	1.2099696660604719E-4
7	0.0	0.0	0.0	4.033232220201573E-5	1.2099696660604719E-4
8	0.0	0.0	0.0	4.033232220201573E-5	1.2099696660604719E-4
9	0.0	0.0	0.0	4.033232220201573E-5	1.2099696660604719E-4
10	0.0	0.0	0.0	4.033232220201573E-5	1.2099696660604719E-4
11	0.0	0.0	0.0	4.033232220201573E-5	1.2099696660604719E-4
12	0.0	0.0	0.0	4.033232220201573E-5	4.8398786642418876E-4
13	0.0	0.0	0.0	4.033232220201573E-5	6.04984833030236E-4
14	0.0	0.0	0.0	4.033232220201573E-5	6.04984833030236E-4
15	0.0	0.0	0.0	8.066464440403146E-5	6.04984833030236E-4
16	0.0	0.0	0.0	8.066464440403146E-5	6.04984833030236E-4
17	4.033232220201573E-5	0.0	0.0	8.066464440403146E-5	6.856494774342673E-4
18	4.033232220201573E-5	0.0	0.0	8.066464440403146E-5	7.259817996362831E-4
19	4.033232220201573E-5	0.0	0.0	1.2099696660604719E-4	7.259817996362831E-4
20	4.033232220201573E-5	0.0	0.0	1.2099696660604719E-4	8.066464440403146E-4
21	4.033232220201573E-5	0.0	0.0	2.823262554141101E-4	8.469787662423303E-4
22	4.033232220201573E-5	0.0	0.0	3.6299089981814154E-4	8.469787662423303E-4
23	4.033232220201573E-5	0.0	0.0	3.6299089981814154E-4	8.469787662423303E-4
24	4.033232220201573E-5	0.0	0.0	4.43655544222173E-4	8.469787662423303E-4
25	8.066464440403146E-5	0.0	0.0	4.43655544222173E-4	8.469787662423303E-4
26	8.066464440403146E-5	0.0	0.0	4.8398786642418876E-4	8.469787662423303E-4
27	8.066464440403146E-5	0.0	0.0	5.243201886262045E-4	8.469787662423303E-4
28	8.066464440403146E-5	0.0	0.0	9.679757328483775E-4	8.469787662423303E-4
29	8.066464440403146E-5	0.0	0.0	0.0015326282436765976	8.87311088444346E-4
30	8.066464440403146E-5	0.0	0.0	0.002097280754504818	8.87311088444346E-4
31	8.066464440403146E-5	0.0	0.0	0.003992899897999557	8.87311088444346E-4
32	8.066464440403146E-5	0.0	0.0	0.005767522074888249	8.87311088444346E-4
33	8.066464440403146E-5	0.0	0.0	0.0077034735405850045	8.87311088444346E-4
34	8.066464440403146E-5	0.0	0.0	0.010446071450322074	8.87311088444346E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1610	0.0	24.27795	1
GTATCAA	3640	0.0	22.85336	1
TATCAAC	5010	0.0	16.140108	2
ATCAACG	5105	0.0	15.715152	3
TCAACGC	5100	0.0	15.6483135	4
CAACGCA	5145	0.0	15.355015	5
AACGCAG	5365	0.0	14.757578	6
CGCACGA	125	2.3220946E-5	14.4037695	35
GTATAGA	460	0.0	13.508502	1
TCTATAC	480	0.0	12.920832	3
CGTGCCG	140	6.776376E-5	12.852987	10
ACGCAGA	6235	0.0	12.538048	7
GTACATG	6205	0.0	12.534102	1
ACCGTGC	385	0.0	12.463503	8
GCACCGT	225	2.5664121E-8	12.440422	6
GTATTAG	355	0.0	12.422175	1
TGCGACT	115	0.0029230698	12.169978	12
TACATGG	6335	0.0	12.100825	2
CGCAGAG	6435	0.0	12.024086	8
GTTTAAG	510	0.0	11.791101	1
>>END_MODULE