FastQCFastQC Report
Mon 6 Feb 2017
SRR2077334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2479401
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.038.453031
GTATCAA45550.036.9205781
TCAACGC61000.027.2718874
ATCAACG61550.027.028743
TATCAAC62800.026.714212
CAACGCA62900.026.4454145
AACGCAG65250.025.702816
ACGCAGA75850.022.1076817
GTACATG59550.021.7773481
CGCAGAG78350.021.2611458
TACATGG59750.021.0003872
AGAGTAC78850.020.16608411
GAGTACT53450.019.61770612
ACATGGG63150.019.154233
CGTGCCG2901.8189894E-1219.0290910
CAGAGTA85150.018.91595610
AGTACTT56850.018.747813
GCAGAGT87650.018.6117739
GTACTTT58950.017.72494114
CGAATCG2156.643677E-817.6411837
Produced by FastQC (version 0.11.5)