FastQCFastQC Report
Mon 6 Feb 2017
SRR2077333_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077333_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164257
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51430.23763351579780034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23300.10765819401300307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.1074271678455932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14000.024.337981
GTATCAA32300.023.331841
TATCAAC42000.017.679712
ATCAACG42550.017.5829473
TCAACGC43050.017.330264
CAACGCA43450.017.2139665
AACGCAG45350.016.532666
GTATACG750.002408039516.0344331
ACGCAGA52500.014.2049037
GAGTACT27550.013.78861912
CGCAGAG54350.013.6846018
CGTACAC1354.7936177E-513.3360843
AGTACTT29450.013.03480913
AGAGTAC53300.012.94138111
CAGAGTA56100.012.68749610
TCGAACT3005.456968E-1212.66313319
GTACTTT29950.012.61751514
GTACTAG2456.157279E-912.2712511
GCAGAGT58500.012.2695139
GTACATG53150.012.1804371
Produced by FastQC (version 0.11.5)