FastQCFastQC Report
Mon 6 Feb 2017
SRR2077333_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077333_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164257
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19250.039.794821
GTATCAA42450.039.194811
TATCAAC61100.027.1088122
TCAACGC61900.026.644024
ATCAACG61950.026.6225193
CAACGCA63000.026.1769855
AACGCAG66200.025.068646
CGAATCG1403.79805E-722.1669537
ACGCAGA76650.021.6518677
CGCAGAG78700.020.9490858
AGAGTAC78600.019.70360611
GAGTACT57200.019.47728312
TAGTCGG1108.269016E-418.80832340
AGTACTT59250.018.57052813
GTACATG51700.018.5047631
CAGAGTA85650.018.44378310
GCAGAGT88700.018.0428949
GTACTTT62350.017.58701514
TACATGG54200.017.4536862
ACATGGG55750.016.5939523
Produced by FastQC (version 0.11.5)