FastQCFastQC Report
Mon 6 Feb 2017
SRR2077332_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077332_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2322146
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71160.30644068030175536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33940.1461579073839457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32740.14099027365204428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.024.7627661
GTATCAA38600.023.7519381
TATCAAC50900.017.7103542
ATCAACG51750.017.484053
TCAACGC52050.017.341834
CAACGCA53000.016.8730225
AACGCAG53650.016.6556766
ACCGTGC3500.015.4212718
GTAACCG1058.468641E-515.25157453
ACGCAGA62750.014.1128417
CGCAGAG63750.013.7660328
ACGCCCG900.00819012313.33592633
GTACATG65250.013.3130491
TATACTG5300.013.2115385
AGAGTAC62150.013.12359511
GAGTACT30750.012.87223312
TCTATAC3750.012.8113223
TACATGG67100.012.7478772
GTATTAC2852.7284841E-1112.6706181
GTAATAC2701.5643309E-1012.6037313
Produced by FastQC (version 0.11.5)