FastQCFastQC Report
Mon 6 Feb 2017
SRR2077332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2322146
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26240.11299892427091149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22250.041.9554441
GTATCAA55750.041.2077751
TATCAAC78150.029.3374312
ATCAACG78400.029.2868523
TCAACGC78650.029.193764
CAACGCA80800.028.2875565
AACGCAG83750.027.4544326
ACGCAGA95700.023.8349887
CGCAGAG98800.023.03838
AGAGTAC97900.022.12519811
GAGTACT68000.021.20203612
CAGAGTA104500.021.12252410
GCAGAGT106950.020.928439
AGTACTT72600.019.95367413
GTACTTT75300.019.27777914
GTACATG62800.019.0645051
TACATGG63350.018.3420472
TACTTTT81900.017.6821615
ACTTTTT88350.016.74395616
ACATGGG68100.016.6544913
Produced by FastQC (version 0.11.5)