FastQCFastQC Report
Mon 6 Feb 2017
SRR2077331_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077331_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304246
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64720.28087278875606164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30510.13240773771550435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29820.12941326577110254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.025.8045371
GTATCAA37050.023.1065351
CGAATCG1401.3260433E-817.14726837
GCGATAC953.635536E-516.83656914
TATCAAC51650.016.4736752
ATCAACG51100.016.4080683
TCAACGC50800.016.3846574
CAACGCA51650.016.0703455
CGCAATT750.002439420416.00342234
AACGCAG53750.015.5108416
TATACCG1101.2677947E-414.5460395
GTGTACG1557.640592E-714.2284021
TAGTCGG850.00560603214.12189240
TTAGTCG1000.001016178514.0042139
CGATACT1303.3782868E-513.84190215
GTACCGA2352.3646862E-1013.6122496
CCGCAAT900.00819492613.33473733
GCACCGT2556.184564E-1113.3286626
TGCGATA1202.661641E-413.3286613
ACGCAGA62100.013.1998827
Produced by FastQC (version 0.11.5)