FastQCFastQC Report
Mon 6 Feb 2017
SRR2077331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304246
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.041.1394581
GTATCAA55650.038.6425671
TCAACGC78100.027.2965414
CAACGCA78750.027.1574945
TATCAAC78500.026.9843462
ATCAACG79050.026.8807953
AACGCAG83150.025.9277176
ACGCAGA94250.022.7668887
CGCAGAG96550.022.0739488
AGAGTAC95100.021.25240111
CAGAGTA101200.020.51533710
GAGTACT66050.020.20826512
GCAGAGT105050.020.0261029
TATCCCG1408.978994E-619.8069255
GTACATG67750.019.6589951
AGTACTT69350.019.44559513
TACATGG69300.019.0604042
GTACTTT69900.018.84442314
ACATGGG70600.018.166893
TAACGCT1150.001075811618.0857664
Produced by FastQC (version 0.11.5)